@article{Villegas Vázquez_Campuzano Ocampo_Hernández Castro_Suárez Güemes_Trigo Tavera_Jaramillo Arango_2014, title={Characterization of capsular types of Pasteurella multocida isolated from clinically healthy beef cattle pharyngeal exudate in the state of Querétaro}, url={https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/415}, DOI={10.22201/fmvz.24486760e.2014.415}, abstractNote={The objective of the present study was to identify and characterize capsular types of <em>P. multocida </em>isolated from beef cattle phar- yngeal exudate in the state of Querétaro. Two hundred and twenty seven pharyngeal exudate swab samples from clinically healthy animals in a slaughterhouse in the municipality of Ezequiel Montes, Querétaro were obtained. Samples were seeded in blood agar and incubated at 37ºC for 24 h under aerobiosis. Strains were identifed through morphological characteristics, conventional biochemical tests and commercial API 2ONE Micro-System. Capsular typing of groups A and D was performed by a multiplex PCR for amplifcation of genes <em>hyaD-hyaC </em>and <em>dcbF</em>, respectively. According to the values established by API WEB software, it was possible to identify 14.09% (32/227) of <em>P. multocida </em>strains, which showed an identifcation percent- age of 96% and a typicality of 1 to <em>P. multocida</em>. By multiplex PCR, the amplifcation of genes <em>hyaD-hyaC, </em>correspondent to capsular group A in 100% (32/32) of the strains previously identifed as <em>P. multocida</em>, was achieved. There are no similar data in Mexico on the identifcation and characterization of <em>P. multocida </em>in beef cattle. With the results obtained it is confrmed that, in a similar way with other countries of Europe and America, capsular type A of <em>P. multocida </em>is predominant in Mexico.}, journal={Veterinaria México OA}, author={Villegas Vázquez, Ana Lilia and Campuzano Ocampo, Víctor Manuel and Hernández Castro, Rigoberto and Suárez Güemes, Francisco and Trigo Tavera, Francisco José and Jaramillo Arango, Carlos Julio}, year={2014}, month={Jul.} }