TY - JOUR AU - Luna del Villar Velasco, Jorge AU - Bernard Bernard, María José AU - Tapia Pérez, Graciela AU - Gutiérrez Olvera, Lilia AU - Sumano López, Héctor PY - 2013/08/26 Y2 - 2024/03/29 TI - Clinical efficacy of sodium alginate meshes for the control of infectious dissemination by Escherichia coli and Staphylococcus epidermidis in cavitated wounds with primary healing JF - Veterinaria México OA JA - Vet Mex OA VL - 43 IS - 4 SE - Original Research Articles DO - UR - https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/322 SP - AB - The potential wound healing ability of sodium alginate (SA) meshes was determined in cavitated rat wounds infected either with <em>Escherichia coli </em>or <em>Staphylococcus epidermidis</em>. Wound progress was evaluated macroscopically and histopathologi­cally to assess dissemination of the induced infection through evaluation of granuloma formation and adjacent tissue ir­ritation. Five groups of 24 female Wistar rats weighing 280 ± 34 g were formed as follows: control group with SA mesh without inoculum, control group with <em>Escherichia coli </em>inoculum without SA mesh, control group with S<em>taphylococcus epi­dermidis </em>inoculum without mesh, group with SA mesh + <em>Escherichia coli </em>inoculum and group with SA mesh + S<em>taphy­lococcus epidermidis </em>inoculum. Abdominal flank incisions were performed under anaesthesia and groups were formed. Both macroscopic and histopathological follow up were carried out and results indicated that the presence of SA meshes avoids or greatly diminishes bacteria dissemination and adjacent tissue irritation. This reaction occurs even in the pres­ence of <em>Escherichia coli </em>or S<em>taphylococcus epidermidis. </em>In the control groups with SA mesh without inoculum and in the groups with <em>Escherichia coli </em>inoculum without SA mesh, and control group with <em>Staphylococcus epidermis </em>inoculum without mesh and group with SA mesh + <em>Escherichia coli </em>inoculum re-epithelialization and wound healing aspect were better than in groups inoculated with S<em>taphylococcus epidermidis</em>, the general odd ratios showed that the dissemination risk was 58 times greater in treatment with SA mesh and <em>E. coli </em>as infectious agent (IC 95% 6.2-210). These results indicate that SA mesh is capable of providing adequate conditions to allow granulation of cavitated wounds, without irritation to adjacent tissues and avoiding dissemination of the infection by <em>E. coli </em>and <em>Staphylococcus epidermidis </em>in rats. However, presence of granuloma can be a healing or esthetic disadvantage, as observed in control group with SA mesh without inoculum. ER -