Evaluation of the degradability of pelagic sargassum under in vitro and in situ conditions for use in ruminant diets
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Abstract
Pelagic sargassum is a mixture of several species of brown macroalgae rich in minerals, polysaccharides, and bioactive compounds; it reaches the Caribbean coasts in large quantities. The objective of this study was to evaluate its potential as an ingredient in ruminant feed based on its in situ degradability and in vitro gas production. 0 %, 10 %, 20 %, and 30 % percentages of sargassum were added to a substrate (Pennisetum purpureum VC CT-115 hay). Two cows with ruminal cannulas were used for the in situ trial, while in vitro gas production was evaluated following the Menke and Steingass methodology. The inoculum was an aliquot of ruminal fluid obtained through an esophageal probe from five grazing hair sheep. In situ degradability of dry matter (DM) decreased linearly (P < 0.0001) as the concentration of sargassum in the substrate increased from 0 % to 30 % (48 h: from 684.91 to 607.75 g/kg DM; 72 h: from 722.10 to 634.68 g/kg DM); organic matter (OM) showed a comparable trend. While the in vitro gas production curve was among the control group, 10 % and 20 % levels of inclusion, degradability of DM and OM, as well as the protozoa population, were did not differ significantly (P > 0.0001) across all treatments. We conclude that pelagic sargassum may be included at levels of 20 % and 30 % in situ and in vitro; however, its use under in vivo conditions requires further evaluation.
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