Phylogenetic analysis and nonsynonymous mutations in a Feline immunodeficiency virus env gene genotype A in Colombian cats Phylogenetic and mutation analysis of FIV env gene in Felix catus
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Abstract
Feline immunodeficiency virus (FIV) is a retrovirus that infects domestic cats. Genetic variability of the virus, particularly in the V3–V5 region of the envelope gene, may contribute to amino acid changes that affect viral pathogenicity and the development of eight distinct subtypes. The circulating genotype and nonsynonymous mutations in an env gene fragment were analyzed in domestic cats from Colombia. Blood samples were collected from 151 felines, and an 859‑base pair fragment of the env gene was amplified and sequenced. Phylogenetic analysis identified genotype A as the circulating genotype; however, sequences clustered into different clades within this genotype. Twelve nonsynonymous amino acid substitutions were detected, of which H86R, N88K, E41V, and K91D showed a high probability of having a deleterious impact. These findings highlight the genetic diversity within FIV genotype A and underscore the potential impact of specific nonsynonymous mutations on viral pathogenicity.
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Kokkinaki KG, Saridomichelakis MN, Leontides L, Mylonakis ME, Konstantinidis AO, Steiner JM, et al. A prospective epidemiological, clinical, and clinicopathologic study of feline leukemia virus and feline immunodeficiency virus infection in 435 cats from Greece. Comparative Immunology, Microbiology and Infectious Diseases. 2021;78:101687. doi.org/10.1016/j.cimid.2021.101687. DOI: https://doi.org/10.1016/j.cimid.2021.101687
Sahay B, Yamamoto JK. Lessons learned in developing a commercial FIV vaccine: the immunity required for an effective HIV-1 vaccine. Viruses. 2018;10(5):277. doi.org/10.3390/v10050277. DOI: https://doi.org/10.3390/v10050277
Sakundech K, Kullaya A, Aengwanich W. Evaluation of the difference between feline immunodeficiency virus (FIV)-infected and healthy cats by using clinicopathological parameters, oxidative stress, and total antioxidant power. Comparative Clinical Pathology. 2021;30(2):149–154. doi.org/10.1007/s00580-021-03222-3. DOI: https://doi.org/10.1007/s00580-021-03222-3
Hosie MJ, Addie D, Belák S, Boucraut-Baralon C, Egberink H, Frymus T, et al. Feline immunodeficiency ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery. 2009;11(7):575–584. doi.org/10.1016/j.jfms.2009.05.006. DOI: https://doi.org/10.1016/j.jfms.2009.05.006
Bęczkowski PM, Hughes J, Biek R, Litster A, Willett BJ, Hosie MJ. Feline immunodeficiency virus (FIV) env recombinants are common in natural infections. Retrovirology. 2014;11(1):80. doi.org/10.1186/s12977-014-0080-1. DOI: https://doi.org/10.1186/s12977-014-0080-1
Teixeira BM, Hagiwara MK, Cruz JCM, Hosie MJ. Feline immunodeficiency virus in South America. Viruses. 2012;4(3):383–396. doi.org/10.3390/v4030383. DOI: https://doi.org/10.3390/v4030383
Safwat MS, Bahr AD, Bakry NM, Amer HM, Yousif AA, Shehata AA, et al. Ancient and dominant: a novel feline immunodeficiency virus subtype "X-EGY" identified in Egyptian cats associated with high prevalence. BMC Veterinary Research. 2025;21(1):497. doi.org/10.1186/s12917-025-04943-1. DOI: https://doi.org/10.1186/s12917-025-04943-1
Bachmann MH, Mathiason-Dubard C, Learn GH, Rodrigo AG, Sodora DL, Mazzetti P, et al. Genetic diversity of feline immunodeficiency virus: dual infection, recombination, and distinct evolutionary rates among envelope sequence clades. Journal of Virology. 1997;71(6):4241–4253. doi.org/10.1128/jvi.71.6.4241-4253.1997. DOI: https://doi.org/10.1128/jvi.71.6.4241-4253.1997
Pecoraro MR, Tomonaga K, Miyazawa T, Kawaguchi Y, Sugita S, Tohya Y, et al. Genetic diversity of Argentine isolates of feline immunodeficiency virus. Journal of General Virology. 1996;77(9):2031–2035. doi.org/10.1099/0022-1317-77-9-2031. DOI: https://doi.org/10.1099/0022-1317-77-9-2031
Teixeira BM, Taniwaki SA, Menezes PMM, Rodrigues AKPP, Mouta AN, Arcebispo TLM, et al. Feline immunodeficiency virus in Northern Ceará, Brazil. Journal of Feline Medicine and Surgery Open Reports. 2019;5(2):2055116919859112. doi.org/10.1177/2055116919859112. DOI: https://doi.org/10.1177/2055116919859112
Biondo D, Kipper D, Maciel JG, de Oliveira Santana W, Streck AF, Lunge VR. Phylogenetic classification of feline immunodeficiency virus. Acta Scientiae Veterinariae. 2023;51. doi.org/10.22456/1679-9216.129530. DOI: https://doi.org/10.22456/1679-9216.129530
Massey Malagón DY, Cuervo Saavedra SR, Lagos López MI. Incidence of immunodeficiency and leukemia viruses in Felis catus in the Veterinary Clinic Gattos Tunja-Boyacá. Ciencia en Desarrollo. 2019;10(1):9–17. doi.org/10.19053/01217488.v10.n1.2019.8402. DOI: https://doi.org/10.19053/01217488.v10.n1.2019.8402
Santisteban-Arenas R, Muñoz-Rodríguez LC, Díaz Nieto J, Pachón Londoño V, Curiel Peña J. Seroprevalencia del virus de inmunodeficiencia felina (VIF) y el virus de la leucemia felina (ViLeF) en gatos del centro de Risaralda, Colombia. Revista de Investigaciones Veterinarias del Perú. 2021;32(3):1-6. doi.org/10.15381/rivep.v32i3.18901. DOI: https://doi.org/10.15381/rivep.v32i3.18901
Arjona A, Barquero N, Doménech A, Tejerizo G, Collado VM, Toural C, et al. Evaluation of a novel nested PCR for the routine diagnosis of feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV). Journal of Feline Medicine and Surgery. 2007;9(1):14–22. doi.org/10.1016/j.jfms.2006.05.009. DOI: https://doi.org/10.1016/j.jfms.2006.05.009
Taniwaki SA, Jiménez-Villegas T, Santana-Clavijo NF, Cruz TF, Silva SOS, Valencia-Bacca JD, et al. Near-complete genome sequence of feline immunodeficiency virus from Colombia. Microbiology Resource Announcements. 2020;9(33):e00754-20. doi.org/10.1128/mra.00754-20. DOI: https://doi.org/10.1128/MRA.00754-20
Kann RK, Kyaw-Tanner MT, Seddon JM, Lehrbach PR, Zwijnenberg RJ, Meers J. Molecular subtyping of feline immunodeficiency virus from domestic cats in Australia. Australian Veterinary Journal. 2006;84(4):112–116. doi.org/10.1111/j.1751-0813.2006.tb13392.x. DOI: https://doi.org/10.1111/j.1751-0813.2006.tb13392.x
Samman A, McMonagle EL, Logan N, Willett BJ, Biek R, Hosie MJ. Phylogenetic characterisation of naturally occurring feline immunodeficiency virus in the United Kingdom. Veterinary Microbiology. 2011;150(3–4):239–247. doi.org/10.1016/j.vetmic.2011.01.027. DOI: https://doi.org/10.1016/j.vetmic.2011.01.027
Zhang J, Wang L, Li J, Kelly P, Price S, Wang C. First molecular characterization of feline immunodeficiency virus in domestic cats from mainland China. PLOS One. 2017;12(1):e0169739. doi.org/10.1371/journal.pone.0169739. DOI: https://doi.org/10.1371/journal.pone.0169739
Roukaerts ID, Theuns S, Taffin ER, Daminet S, Nauwynck HJ. Phylogenetic analysis of feline immunodeficiency virus strains from naturally infected cats in Belgium and The Netherlands. Virus Research. 2015;196:30–36. doi.org/10.1016/j.virusres.2014.10.023. DOI: https://doi.org/10.1016/j.virusres.2014.10.023
Taniwaki SA, Figueiredo AS, Araujo JP Jr. Virus-host interaction in feline immunodeficiency virus (FIV) infection. Comparative Immunology, Microbiology and Infectious Diseases. 2013;36(6):549–557. doi.org/10.1016/j.cimid.2013.07.001. DOI: https://doi.org/10.1016/j.cimid.2013.07.001
Motokawa K, Hohdatsu T, Imori A, Arai S, Koyama H. Mutations in feline immunodeficiency (FIV) virus envelope gene V3-V5 regions in FIV-infected cats. Veterinary Microbiology. 2005;106(1–2):33–40. doi.org/10.1016/j.vetmic.2004.12.016. DOI: https://doi.org/10.1016/j.vetmic.2004.12.016
Luttge BG, Panchal P, Puri V, Checkley MA, Freed EO. Mutations in the feline immunodeficiency virus envelope glycoprotein confer resistance to a dominant-negative fragment of Tsg101 by enhancing infectivity and cell-to-cell virus transmission. Biochimica et Biophysica Acta–Biomembranes. 2014;1838(4):1143–1152. doi.org/10.1016/j.bbamem.2013.08.020. DOI: https://doi.org/10.1016/j.bbamem.2013.08.020
Wyatt R, Sodroski J. The HIV-1 envelope glycoproteins: fusogens, antigens and immunogens. Science. 1998;280(5371):1884–1888. doi.org/10.1126/science.280.5371.1884. DOI: https://doi.org/10.1126/science.280.5371.1884
Doms RW, Moore JP. HIV-1 membrane fusion: targets of opportunity. Journal of Cell Biology. 2000;151(2):F9–F14. doi.org/10.1083/jcb.151.2.F9. DOI: https://doi.org/10.1083/jcb.151.2.F9
Jackson T, King AM, Stuart DI, Fry E. Structure and receptor binding. Virus Research. 2003;91(1):33–46. doi.org/10.1016/s0168-1702(02)00258-7. DOI: https://doi.org/10.1016/S0168-1702(02)00258-7
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