Inmunoprofilaxis contra la infección por Salmonella enteritidis en pollos de engorda mediante el uso de linfocinas

Abstract

BROILER CHICKS WERE TREATED PROPHYLACTICALLY WITH THE SOLUBLE PRODUCTS FROM CONCANAVALIN ASTIMULATED T-LYMPHOCYTES FROM EIMERIA TENELLA -INFECTED CHICKENS IN ORDER TO STUDY THE EFFECT OF SUCH PROPHYLACTIC TREATMENT ON ORGAN INVASION BY SALMONELLA ENTERITIDIS. CHICKS WERE RANDOMLY ASSIGNED IN THREE GROUPS: GROUP A) EXPERIMENTAL GROUP (S. ENTERITIDIS-INFECTED, TREATED): AT ONE DAY OF AGE, CHICKS WERE INJECTED INTRAPERITONEALLY WITH E. TENELLA-IMMUNE LYMPHOKINES, AND THIRTY MINUTES AFTER THE LYMPHOKINE INJECTION ALL CHICKS WERE CHALLENGED PER OS WITH 105 COLONY-FORMING UNITS OF S. ENTERITIDIS; GROUP B) THE POSITIVE CONTROL GROUP (S. ENTERITIDIS-INFECTED, NOT TREATED) AND GROUP C) THE NEGATIVE CONTROL GROUP (S. ENTERITIDIS-NOT INFECTED, NOT TREATED). AT 24 HOURS POSTCHALLENGE, ALL CHICKS WERE EUTHANIZED; LIVER, SPLEEN AND CECAL TONSILS WERE COLLECTED. ISOLATION OF S. ENTERITIDIS WAS ATTEMPTED FROM THESE TISSUES. THE TREATMENT OF CHICKS WITH LYMPHOKINES RESULTED IN REDUCTION OF S. ENTERITIDIS ISOLATION FROM LIVER-SPLEEN (P<0.05) BUT NOT FROM CECAL TONSILS (P>0.05). THESE RESULTS DEMONSTRATE THAT THE PROPHYLACTIC TREATMENT WITH E. TENELLA-IMMUNE LYMPHOKINES REDUCES THE S. ENTERITIDIS ORGAN INVASION BUT NOT THE INTESTINAL COLONIZATION IN NEONATAL BROILER CHICKS.