Proteins from Mycobacterium bovis culture filtrate promote apoptosis independently of caspase activation in bovine macrophages
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Abstract
Apoptosis is a normally occurring process characterized by morphological changes in the cell. The species of the Mycobacterium tuberculosis complex induce apoptosis. In bovines, Mycobacterium bovis and its protein extracts induce apoptosis independently of caspase activation. However, the identity of the proteins in the extracts is unknown. In this study, we used size-exclusion chromatography to separate protein fractions from the culture filtrate. We obtained protein fractions that induced DNA fragmentation in bovine macrophages independently of caspase-3 activation. Liquid chromatography-mass spectrometry (LC-MS) identified three candidates responsible for the biological activity: MPB70, MPB83, and 60 kDa chaperonin.
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References
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