Serum lipopolysaccharide as a diagnostic and prognostic biomarker in Canis familiaris pyometra
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Specific sensitive diagnostic markers for early diagnosis of canine pyometra have been widely attempted, but none have proven to be a perfect diagnostic marker for clinical practice. Lipopolysaccharide (LPS) or endotoxin is a cell wall component of gram-negative bacteria, released during bacterial death. The early release, inherent stability, and serotype-specificity of endotoxin suggest its potential as a good candidate for early diagnosis of pyometra. The present study focused on understanding the potential of LPS as a diagnostic and prognostic marker in canine pyometra. The study also tried to establish the correlation between bacterial isolates cultured from the vaginal discharge and endotoxin levels. A total of 12 animals presented to University Veterinary Hospitals with open and closed-cervix pyometra were selected and divided into two groups of six dogs each. Serum LPS concentration (ng/mL) was estimated on various days of observation using ELISA. Mean serum LPS concentration was higher in both groups on the day of presentation, and significantly higher levels were observed in animals with closed-cervix pyometra. Bacteriological examination revealed a predominance of gram-negative organisms in both groups. Hence, it establishes LPS as a better diagnostic marker of canine pyometra. Conversely, a reduction in the levels of LPS on subsequent days of observation confirms its efficacy as a prognostic marker.
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Mona N, Reza NS, Sahar GK, Farzad H. Effects of isoflavone supplementation on endometrial thickness, endometrial hyperplasia, and cancer in ovariectomized cats. Veterinaria Italiana. 2022;58(1):35–39. doi: 10.12834/vetit.1741.9188.2.
Nayana D, Becha BB, Jayakumar C, Unnikrishnan MP, Venugopal SK. Occurrence of pyometra among dogs —a retrospective study. Journal of Indian Veterinary Association. 2024;22(3):100–107. doi: 10.55296/JIVA/22.3.2024. DOI: https://doi.org/10.55296/JIVA/22.3.2024.100-107
Nayana D, Becha BB, Jayakumar C, Unnikrishnan MP, Venugopal SK. Haemato-biochemical studies in medically managed open and closed-cervix pyometra in dogs. Journal of Veterinary and Animal Sciences. 2021;52(3):281–285. doi: 10.51966/jvas.2021.52.3.281-285. DOI: https://doi.org/10.51966/jvas.2021.52.3.281-285
Liao AT, Huang WH, Wang SL. Bacterial isolation and antibiotic selection after ovariohysterectomy of canine pyometra: a retrospective study of 55 cases. Taiwan Veterinary Journal. 2020;46(1):1–8. doi: 10.1142/S1682648520500067. DOI: https://doi.org/10.1142/S1682648520500067
Hardy RM, Osborne CA. Canine pyometra: Pathophysiology, diagnosis, and treatment of uterine and extra-uterine lesions. Journal of the American Animal Hospital Association. 1974;10(3):245–268.
Sandholm M, Vasenius H, Kivisto AK. Pathogenesis of canine pyometra. Journal of the American Veterinary Medical Association. 1975;167(11):1006–1010. https://europepmc.org/article/med/1104545 DOI: https://doi.org/10.2460/javma.1975.167.11.1006
Crutchley MJ, Marsh DG, Cameron J. Free endotoxin. Nature. 1967;214:1052. doi: 10.1038/2141052a0. DOI: https://doi.org/10.1038/2141052a0
Okoko T, Faith OR. Inhibitory potential of rutin on lipopolysaccharide-induced toxicity and inflammatory response of raw U937 cells and macrophages. Notulae Scientia Biologicae. 2022;14(3):11294. doi: 10.55779/nsb14311294. DOI: https://doi.org/10.55779/nsb14311294
Cohen J. The detection and interpretation of endotoxaemia. Intensive Care Medicine. 2000;26(Suppl 1): S051–S056. doi: 10.1007/s001340051119. DOI: https://doi.org/10.1007/s001340051119
Jitpean S, Ambrosen A, Emanuelson U, Hagman R. Closed cervix is associated with more severe illness in dogs with pyometra. BMC Veterinary Research. 2016;13:11. doi: 10.1186/s12917-016-0924-0. DOI: https://doi.org/10.1186/s12917-016-0924-0
Ceron JJ, Pardo-Marin L, Wdowiak A, Zoia A, Wochnik M, Szczubial M, et al. Divergences between serum C-reactive protein and ferritin concentrations in canine pyometra. BMC Veterinary Research. 2023;19: 78. doi: 10.1186/s12917-023-03630-3. DOI: https://doi.org/10.1186/s12917-023-03630-3
Okano S, Tagawa M, Takase K. Relationship of the blood endotoxin concentration and prognosis in dogs with pyometra. Journal of Veterinary Medical Science. 1998;60(11):1265–1267. doi: 10.1292/jvms.60.1265. DOI: https://doi.org/10.1292/jvms.60.1265
Takahashi K, Fukuda M, Kawai M, Yokochi T. Detection of lipopolysaccharide (LPS) and identification of its serotype by an enzyme-linked immunosorbent assay using polyl-L-lysine. Journal of Immunological Methods. 1992;153(1–2): 67–71. doi: 10.1016/0022-1759(92)90306-E. DOI: https://doi.org/10.1016/0022-1759(92)90306-E
Lansubsakul N, Sirinarumitr K, Sirinarumitr T, Imsilp K, Wattananit P, Supanrung S. First report on clinical aspects, blood profiles, bacterial isolation, antimicrobial susceptibility, and histopathology in canine pyometra in Thailand. Veterinary World. 2022;15(7):1804–1813. doi: 10.14202/vetworld.2022.1804–1813. DOI: https://doi.org/10.14202/vetworld.2022.1804-1813
Pailler S, Slater MR, Lesnikowski SM, Gayle JM, Duvieusart CB, Ledesma EJ, et al. Findings and prognostic indicators of outcomes for bitches with pyometra treated surgically in a nonspecialized setting. Journal of the American Veterinary Medical Association. 2022;260(S2): 5–7. doi: 10.2460/javma.20.12.0713. DOI: https://doi.org/10.2460/javma.20.12.0713
Wang M, Feng J, Zhou D, Wang J. Bacterial lipopolysaccharide-induced endothelial activation and dysfunction: A new predictive and therapeutic paradigm for sepsis. European Journal of Medical Research. 2023;28:339. doi: 10.1186/s40001-023-01301-5. DOI: https://doi.org/10.1186/s40001-023-01301-5
Snedecor GW, Cochran WG. Statistical Methods. 8th edition. Iowa, US: The Iowa State University Press; 1991. 503 pp.
Hagman R. New aspects of canine pyometra: studies on epidemiology and pathogenesis [doctoral thesis dissertation]. Uppsala, SE: Sverigeslantbruksuniversitet (Swedish University of Agricultural Sciences); 2004.
Alexander C, Rietschel ET. Bacterial lipopolysaccharides and innate immunity. Journal of Endotoxin Research. 2001;7(3):167–202. doi: 10.1177/0968051901007003010. DOI: https://doi.org/10.1179/096805101101532675
Dawson ME. The significance of endotoxin to cell culture and biotechnology (PDF). LAL Update 1998;16(1). https://www.acciusa.com/pdfs/newsletter/updt0398.pdf
Fransson B, Lagerstedt AS, Hellmen E, Jonsson P. Bacteriological findings, blood chemistry profile, and plasma endotoxin levels in bitches with pyometra or other uterine diseases. Journal of Veterinary Medicine Series A. 1997;44(7):417–426. doi: 10.1111/j.1439-0442.1997.tb01127.x. DOI: https://doi.org/10.1111/j.1439-0442.1997.tb01127.x
Peterson SL, Koblik PD, Whiting PG, Breznock EM. Endotoxin concentrations measured by a chromogenic assay in portal and peripheral venous blood in ten dogs with portosystemic shunts. Journal of Veterinary Internal Medicine. 1991;5(2):71–74. doi: 10.1111/j.1939-1676.1991.tb00934.x. DOI: https://doi.org/10.1111/j.1939-1676.1991.tb00934.x
Johnston SD, Kustritz MVR, Olson PNS. Canine and feline theriogenology. 2nd edition. Philadelphia, US: WB Saunders Company; 2001. 592 pp.
Ucmak M, Tek C, Gunduz MC, Sabuncu A, Senunver A, Bagcigil FA, Bakirel T. Optimum timing for operation in bitches with pyometra related to endotoxemia. Turkish Journal of Veterinary and Animal Sciences. 2012;36(1):35–42. doi: 10.3906/vet-1102-762. DOI: https://doi.org/10.3906/vet-1102-762
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