Interference of a hemagglutinant fraction of crotalic venom in the adsortion of parainfluenza-3 (PI-3) virus

Abstract

AN ISOLATED PROTEIN FRACTION OF VENOM OF AGKISTRODON PISCIVORUS WITH HEMAGGLUTINANT PROPERTY WAS EVALUATED IN ITS CAPACITY TO INHIBIT IN VITRO THE BOVINE PARAINFL UENZA-3 (PI3) VIRUS, EITHER BY ITS CAPACITY TO BLOCK CELL RECEPTORS OR ITS ADHERENCE TO THE HEMAGGLUTININ VIRAL SPICULES. FRACTION AL27 ACTING AS A RECEPTOR BLOCK, AT A CONCENTRATION 1.062 Å“G/ML, INHIBITED ANY DAMAGE OR DESTRUCTION OF MADIN DARBY BOVINE KIDNEY (MDBK) CELL CULTURES BY THE PI-3 VIRUS (TITER 105.6 TCID50%), MAINTAINING A CELL VIABILITY BETWEEN 69.43 AND 84.86%. THE ADHERENCE CAPACITY OF AL27 TO VIRAL HEMAGGLUTININ SPICULES OF PI3 VIRUS WAS DETERMINED BY INCUBATING BOTH REACTANTS 1:1 (1 ML AL27 AT CONCENTRATION 1.062 Å“G/ML 1 ML PI-3 VIRUS TITER 105.6 DICC50%) AT 37°C FOR 1 HOUR, AND A PROBABLE ADHERENCE TO THE VIRAL HEMAGGLUTININ SPICULES WAS DETECTED BY ELECTRON MICROSCOPE. THE INACTIVATION OF PI-3 VIRUS BY AL27 WAS DEDUCED BY THE REDUCTION OF THE VIRAL TITER FROM 105.6 TO 102.0 TCID50%. THE APPARENT IDENTITY BETWEEN AL27 AND PI-3 VIRUS WAS DETERMINED BY RECOGNITION OF THE COMMON PROTEIN 21 KDA WHEN PERFORMING A WESTERN BLOT USING HYPER-IMMUNE ANTI- AGKISTRODON PISCIVORUS SERUM. THIS OUTCOME SHOWS THE POSSIBILITY OF USING AL27 FRACTION AS AN ANTIVIRAL AGENT.