Protective effect of thiamine pyrophosphate in the hippocampus of rats exposed to stress by forced immobilization

Abstract

THE ABILITY OF THIAMINE PYROPHOSPHATE (TPP) OR ACTIVATED VITAMIN B1 IN PREVENTING NEURONAL DAMAGE TO THE RAT HIPPOCAMPUS EXPOSED TO STRESS BY FORCED IMMOBILIZATION WAS APPRAISABLE ON THIS STUDY. TWENTY ONE DAYS OLD, MALE RATS WERE USED AND DISTRIBUTED ON FOUR GROUPS OF TEN RATS EACH: CONTROL (C), CHRONIC STRESS (CS), CHRONIC STRESS TPP (CS TPP), AND TPP (TPP). THE ANALYZED VARIABLES WERE: BODY WEIGHT, DENDRITIC APICAL LENGTH, MAJOR AND MINOR NEURONAL SOMA AXES, AS WELL AS THE AVERAGE OF DENDRITIC BRANCHES AND SPINES. THE RESULTS SHOWED SIGNIFICANT DECREASES ON BODY WEIGHT (FROM 20 TO 40%) IN ALL GROUPS WHEN COMPARING WITH C GROUP, AND A STATISTICALLY SIGNIFICANT DECREASE AT SERUM CORTICOSTERONE LEVELS OF 20.52% IN TPP AND 16.3% IN CS TPP GROUPS WHEN COMPARING WITH C GROUP. A SIGNIFICANT INCREASE WAS FOUND (6.5%) ON DIAMETER OF MINOR NEURONAL SOMA AXIS IN CS TPP GROUP COMPARED TO C GROUP. OTHER STATISTICALLY SIGNIFICANT DECREASES WERE: DENDRITIC LENGTH OF 32.1% IN CS GROUP COMPARED TO C, 27.2% IN CS GROUP IN RELATION TO CS TPP AND 30.2% IN CS GROUP COMPARED TO ONLY TPP SUPPLIED GROUP. FINALLY, THE NUMBER OF DENDRITIC SPINES SHOWED STATISTICALLY SIGNIFICANT DECREASE OF 59.4% IN RELATION TO CS GROUP, 50.8% IN CS GROUP COMPARED TO CS TPP GROUPS, AND 57.7% IN GROUP CS COMPARED TO TPP SUPPLIED GROUP. ACCORDING TO THESE RESULTS, TPP ACTS PREVENTING THE INCREASE OF SERUM CORTICOSTERONE LEVELS IN STRESSED RATS SUPPLIED WITH TPP WHICH PRESERVES NEURONAL INTEGRITY ON THE ANALYZED VARIABLES, SUCH AS DENDRITIC LENGTH AND DENDRITIC SPINES AVERAGE.THIAMINE P