Determination of Salmonella Enteritidis FT 13 A and Salmonella Issatschenko by PCR in samples of chicks experimentally infected

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Israel Monroy Becerra
Néstor Ledesma Martínez
Félx Domingo Sánchez Godoy
Griselda Ruiz Flores
Odette Urquiza Bravo

Abstract

The objective of this work was to determine by PCR the presence of Salmonella Enteritidis FT-13A (SE) and Salmonella Issats­chenko (SI) in different samples of organs from 4 days old chicks experimentally infected. Four days old chicks were inoculated with SE and SI and their organs were frozen for DNA extraction to perform classic and nested PCR. It was possible to demonstrate the presence of SE and SI after 6 hours after experimental infection (AEI), but as time passed AEI positive results were incon­sistent, obtaining negative results until 174 hours AEI. PCR is useful for detecting SE and SI in the early hours AEI. It is recom­mended to use pre-enrichment of the samples, in order to facilitate the DNA extraction and the detection of Salmonella by PCR


Keywords: Classic PCR, Nested PCR, Salmonella Enteritidis, Salmonella Issatschenko, Chicks,

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