Characterization of capsular types of Pasteurella multocida isolated from clinically healthy beef cattle pharyngeal exudate in the state of Querétaro

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Ana Lilia Villegas Vázquez
Víctor Manuel Campuzano Ocampo
Rigoberto Hernández Castro
Francisco Suárez Güemes
Francisco José Trigo Tavera
Carlos Julio Jaramillo Arango

Abstract

The objective of the present study was to identify and characterize capsular types of P. multocida isolated from beef cattle phar- yngeal exudate in the state of Querétaro. Two hundred and twenty seven pharyngeal exudate swab samples from clinically healthy animals in a slaughterhouse in the municipality of Ezequiel Montes, Querétaro were obtained. Samples were seeded in blood agar and incubated at 37ºC for 24 h under aerobiosis. Strains were identifed through morphological characteristics, conventional biochemical tests and commercial API 2ONE Micro-System. Capsular typing of groups A and D was performed by a multiplex PCR for amplifcation of genes hyaD-hyaC and dcbF, respectively. According to the values established by API WEB software, it was possible to identify 14.09% (32/227) of P. multocida strains, which showed an identifcation percent- age of 96% and a typicality of 1 to P. multocida. By multiplex PCR, the amplifcation of genes hyaD-hyaC, correspondent to capsular group A in 100% (32/32) of the strains previously identifed as P. multocida, was achieved. There are no similar data in Mexico on the identifcation and characterization of P. multocida in beef cattle. With the results obtained it is confrmed that, in a similar way with other countries of Europe and America, capsular type A of P. multocida is predominant in Mexico.

Keywords: Pasteurella multocida, capsular types, biochemical characterization, molecular characterization, pharyngeal exudate, cattle, PCR

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