Characterization of capsular types of Pasteurella multocida isolated from clinically healthy beef cattle pharyngeal exudate in the state of Querétaro

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Ana Lilia Villegas Vázquez
Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México
Víctor Manuel Campuzano Ocampo
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México
Rigoberto Hernández Castro
Dirección de Investigación, Hospital General "Dr. Manuel Gea González", Secretaria de Salud
Francisco Suárez Güemes
Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México
Francisco José Trigo Tavera
Departamento de Patología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México
Carlos Julio Jaramillo Arango
Centro de Enseñanza, Investigación y Extensión en Producción Animal en Altiplano CEIEPAA, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México

Abstract

The objective of the present study was to identify and characterize capsular types of P. multocida isolated from beef cattle phar- yngeal exudate in the state of Querétaro. Two hundred and twenty seven pharyngeal exudate swab samples from clinically healthy animals in a slaughterhouse in the municipality of Ezequiel Montes, Querétaro were obtained. Samples were seeded in blood agar and incubated at 37ºC for 24 h under aerobiosis. Strains were identifed through morphological characteristics, conventional biochemical tests and commercial API 2ONE Micro-System. Capsular typing of groups A and D was performed by a multiplex PCR for amplifcation of genes hyaD-hyaC and dcbF, respectively. According to the values established by API WEB software, it was possible to identify 14.09% (32/227) of P. multocida strains, which showed an identifcation percent- age of 96% and a typicality of 1 to P. multocida. By multiplex PCR, the amplifcation of genes hyaD-hyaC, correspondent to capsular group A in 100% (32/32) of the strains previously identifed as P. multocida, was achieved. There are no similar data in Mexico on the identifcation and characterization of P. multocida in beef cattle. With the results obtained it is confrmed that, in a similar way with other countries of Europe and America, capsular type A of P. multocida is predominant in Mexico.
Keywords:
Pasteurella multocida capsular types biochemical characterization molecular characterization pharyngeal exudate cattle PCR
DOI: https://doi.org/10.22201/fmvz.24486760e.2014.415

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References

Cooper VL, Brodersen BW. Bovine Respiratory Disease. Vet Clin North Am: Food Anim Pract 2010; 26:191-426.

Lilie LE. The Bovine Respiratory Disease Complex. The Canadian Veterinary Journal. Symposium on Immunization of Cattle Against the Common Diseases of the Respiratory Tract. Rev Vet Can 1974; 15:233-42.

Jaramillo ACJ, Trigo TFJ, Suárez GF, Mannheimiosis Bovina: Etiología, Prevención y Control. Vet Méx 2009; 403:293-311.

Boyce RY, Wilkie I, Adler B. Pasteurella and Mannheimia In: GYLES CL, PRESCOTT JF. Pathogenesis of bacterial infections in animals. 3rd ed. Iowa: Blackwell Publishing 2007; 1: 273-270.

Vidhya M, Chandran DJ, Manobar P, Dhinakar R. Molecular identification of serogroups of Pasteurella multocida isolated from sheep by capsular PCR typing. J Vet Anim Sci 2007;3:140-143.

Cueto LM, Pascual HA. Pasteurella multocida. Departamento de microbiología. Hospital universitario Virgen Macarena. Sevilla. [En línea: 2013 enero] [Citado: 2013 enero 20]. Disponible en: http:// www.seimc.org/control/revisiones/bacteriologia/ pmultocida.pdf

Boyce JD. Jing YC. Adler B. Pasteurella multocida capsule: composition, function and genetics. J Biotechnol 2000; 83:153-160.

Campuzano O VM, González RAD, Hernández CR, Suárez GF, Trigo TFJ, Jaramillo ACJ. Caracterización fenotípica y molecular de cepas de Pasteurella multocida de exudado nasal en bovinos, en dos cuencas lecheras de México. Vet Méx 2011; 1:1-10.

Boyce JD, Cheng J Y, Adler B. Genetic organization of the capsule biosynthetic locus of Pasteurella multocida M104 (B:2). Vet Microbiol 2000; 72:121-34.

Jaramillo ACJ, Hernández CR, Suárez GFS, Martínez MJ, Aguilar RF, Jaramillo ML et al. Characterization of Mannheimia spp.strains isolated from bovine nasal exudate and factors associated with isolates, in dairy farms in the Central Valley of Mexico. Res Vet Sci 2008; 84: 7-13.

Szabo I, Uda H, Sakamoto H. Rapid techniques for DNA extraction from routinely processed archival tissue for use in PCR. Clin Pathol 1994; 47:318-323.

Rajeev G, Kumar A, Singh VP, Vijendra P. Singh,T.K. Dutta, SB. Specific identification of Pasteurella multocida serogroup-A isolates by PCR assay. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Veterinary science 2004; 76:179-185.

De Rosa DC, Mechor GD, Staats JJ, Chengappa MM. Comparison of Pasteurella spp simultaneously Isolated from Nasal and Transtracheal Swabs from Cattle with Clinical Signs of Bovine Respiratory Disease. Elanco Animal Health, Greenfield, Indiana 46140,1 and Kansas State University. J Clin Microbiol 2000; 38:327-332.

Gourlay RN, Thomas LH, Wyld SG. Experimental Pasteurella multocida pneumonia in calves. Res Vet Sci 1989; 47:185-189.

Virtala AMK, Mechor GD, Grohn YT, Erb HN, Dubovi EJ. Epidemiologic and pathologic characteristics of respiratory tract disease in dairy heifers during the first three months of life. J Am Vet Med Assoc 1996; 208:2035-2042.

Ames TR. Dairy calf pneumonia. Vet Clin North AmFood Anim Pract 1997; 13:379-391.

Trigo TF, Hernández LG, Ramírez CC, Berruecos VM. Patología y bacteriología de pulmones neumónicos de becerros. Vet Méx 1982; 13:131-140.

Blanco-Viera FJ, Trigo FJ, Jaramillo ML, Aguilar RF. Serotypes of Pasteurella multocida and Pasteurella haemolytica isolated from pneumonic lesions in cattle and sheep from Mexico. Rev Lat-Amer Microbiol 1995; 37:121-126.

Jaramillo ACJ, Hernandez CR, Campuzano OV, Suárez GR, Delgado GR, Trigo TF. Characterization of Mannheimia sp and P.multocida strains isolated from bovine pneumonic lungs in two slaughterhouses in Mexico. J Anim Vet Adv 2007; 12:1384-1403.

Dabo SM, Debey BM, Montelongo M, Confer W. Genomic DNA restriction site heterogeneity in bovine Pasteurella multocida serogroup A isolates detected wth rRNA probe. J. Med Microbiol 1999; 48: 279-286.

Collins MT, Weaver N, Ellis RP. Identification of Pasteurella multocida and Pasteurella haemolytica by API 2ONE, Minitek and Oxy/ferm Systems. J Clin Microb 1981; 13:433-437.

Townsend KM, Boyce JD, Yeng CJ, Frost AJ, Adler B. Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR Typing System. J Clin Microbiol 2001; 39:924-929.

De La Rosa RJL, Jaramillo ACJ, Martínez MJJ, Agilar RF, Hernández CR, Suárez GF et al. Frecuencia de aislamientos de Mannheimia haemolytica y Pasteurella multocida en becerras con signos clínicos de enfermedad respiratoria, en un complejo lechero del estado de Hidalgo, México. Vet Méx 12; 43:1-10.

Jaramillo CL, Aguilar RF, Trigo TF. Serotipificación de Pasteurella haemolytica determinación de los tipos capsulares de Pasteurella multocida aisladas de pulmones neumónicos de becerros en México. Vet Méx 1987; 18:185-188.

Yates WDG. A review of infectious bovine rhynotracheitis, shipping fever pneumonia and viralbacterial synergism in respiratory disease of cattle. Can J Comp Med 1982; 46:225-263

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