<em>Mycobacterium tuberculosis</em> and <em>Mycobacterium bovis</em> derived proteins induce caspase-independent apoptosis in bovine macrophages

Main Article Content

Anaid Maciel-Rivera
Susana Flores-Villalva
Itzel Jímenez-Vázquez
Omar Catalán-Barcenas
Clara Inés Espitia-Pinzón
Julio Morán
Lourdes Arriaga-Pizano
Alejandro Benítez-Guzmán
Edgar Alfonseca-Silva
José Ángel Gutiérrez-Pabello

Abstract

Veterinaria México OA
ISSN: 2448-6760

Cite this as:

  • Maciel-Rivera A, Flores-Villalva S, Jímenez-Vázquez I, Catalán-Barcenas O, Espitia-Pinzón CI, Morán J, Arriaga-Pizano L, Benítez-Guzmán A, Alfonseca-Silva E, Gutiérrez-Pabello JA. Mycobacterium tuberculosis and Mycobacterium bovis derived proteins induce caspase-independent apoptosis in bovine macrophages. Veterinaria México OA. 2019;6(1). doi: 10.22201/fmvz.24486760e.2019.1.560.
Species of the genus Mycobacterium are capable of inducing cell apoptosis. Infected cells with M. tuberculosis undergo apoptosis through a caspase-dependent pathway. We have previously shown that whole Mycobacterium bovis cells and derived crude protein extracts were able to trigger apoptosis through a caspase independent mechanism. However, the identity of the protein or protein fractions capable of inducing apoptosis has not been determined. In this study, bovine macrophages were incubated in the presence of Mycobacterium tuberculosis recombinant proteins and Mycobacterium bovis protein extracts to identify proteins capable of inducing apoptosis. A subgroup of proteins and protein extracts from strains of the Mycobacterium tuberculosis complex were shown to induce DNA fragmentation in bovine macrophages through a caspase independent pathway. Our findings suggest that bovine macrophages may elicit a different response to mycobacterium infection than that displayed by murine or human cells.
Keywords:
M. bovis M. tuberculosis bovine macrophages caspase

Article Details

Author Biographies

Susana Flores-Villalva, Centro Nacional de Investigación Disciplinaria (CENID) Fisiología, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Querétaro, México

Investigador Titular B

José Ángel Gutiérrez-Pabello, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, México

Profesor de Titular C de Tiempo Completo, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México.

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