<em>Mycobacterium tuberculosis</em> and <em>Mycobacterium bovis</em> derived proteins induce caspase-independent apoptosis in bovine macrophages

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Anaid Maciel-Rivera
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, México
Susana Flores-Villalva
Centro Nacional de Investigación Disciplinaria (CENID) Fisiología, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Querétaro, México
Itzel Jímenez-Vázquez
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, México
Omar Catalán-Barcenas
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, México
Clara Inés Espitia-Pinzón
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México, México, México
Julio Morán
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, México, México
Lourdes Arriaga-Pizano
Unidad de Investigación Médica en Inmunoquímica, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México, México
Alejandro Benítez-Guzmán
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, México
Edgar Alfonseca-Silva
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, México
José Ángel Gutiérrez-Pabello
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, México

Resumen

Veterinaria México OA
ISSN: 2448-6760

Cite this as:

  • Maciel-Rivera A, Flores-Villalva S, Jímenez-Vázquez I, Catalán-Barcenas O, Espitia-Pinzón CI, Morán J, Arriaga-Pizano L, Benítez-Guzmán A, Alfonseca-Silva E, Gutiérrez-Pabello JA. Mycobacterium tuberculosis and Mycobacterium bovis derived proteins induce caspase-independent apoptosis in bovine macrophages. Veterinaria México OA. 2019;6(1). doi: 10.22201/fmvz.24486760e.2019.1.560.
Species of the genus Mycobacterium are capable of inducing cell apoptosis. Infected cells with M. tuberculosis undergo apoptosis through a caspase-dependent pathway. We have previously shown that whole Mycobacterium bovis cells and derived crude protein extracts were able to trigger apoptosis through a caspase independent mechanism. However, the identity of the protein or protein fractions capable of inducing apoptosis has not been determined. In this study, bovine macrophages were incubated in the presence of Mycobacterium tuberculosis recombinant proteins and Mycobacterium bovis protein extracts to identify proteins capable of inducing apoptosis. A subgroup of proteins and protein extracts from strains of the Mycobacterium tuberculosis complex were shown to induce DNA fragmentation in bovine macrophages through a caspase independent pathway. Our findings suggest that bovine macrophages may elicit a different response to mycobacterium infection than that displayed by murine or human cells.
DOI: https://doi.org/10.22201/fmvz.24486760e.2019.1.560

Detalles del artículo

Biografía del autor/a

Susana Flores-Villalva, Centro Nacional de Investigación Disciplinaria (CENID) Fisiología, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Querétaro, México

Investigador Titular B

José Ángel Gutiérrez-Pabello, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, México

Profesor de Titular C de Tiempo Completo, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México.

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