Identification of Mycobacterium bovis infected cattle by immunological and molecular methods
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Abstract
DIAGNOSIS OF BOVINE TUBERCULOSIS (TB) IS USUALLY PERFORMED BY THE TUBERCULIN INTRADERMAL TEST, WHICH ALLOWS DETECTION OF CATTLE THAT HAVE BEEN EXPOSED TO MYCOBACTERIUM BOVIS. HOWEVER, IN HERDS WHERE CONTROL OF TB IS BASED ON THE IDENTIFICATION AND REMOVAL OF REACTORS TO THIS TEST, SOME ANIMALS IN ADVANCED STAGES OF THE DISEASE AND WITH OPEN LESIONS DO NOT SHOW REACTIVITY TO TUBERCULIN (ANERGIC), AND MIGHT REMAIN IN THE HERD, THUS CONSTITUTING A POTENTIAL SOURCE OF INFECTION IN SUSCEPTIBLE CATTLE. THE OBJECTIVE OF THIS STUDY WAS TO USE ALTERNATIVE METHODS OF DIAGNOSIS TO IDENTIFY POSITIVE TB BOVINES THAT ARE NOT DETECTED BY THE INTRADERMAL TEST. A DAIRY HERD OF 277 BOVINES, WHICH HAD BEEN UNDER SUPERVISION BY THE NATIONAL TB CONTROL AND ERADICA
Keywords:
BOVINE TUBERCULOSIS DIAGNOSIS ELISA IFN-G SPOLIGOTYPING DAIRY CATTLE
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