Acute toxicity assessment and subacute of compound alpha injectable (5-chloro-2-(methylthio)-6-(1- naphthyloxy)-1H-benzimidazole) in rats
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Abstract
The objective of this work was to evaluate the acute and subacute toxicity of the injectable alpha compound to Hsd Han: WIST rats. For the acute toxicity assessment, rats were inoculated with doses of 2000, 1000, 500, 100, or 10mg/kg alpha compound intramuscularly. The results of histopathological analysis did not show apparent changes with the doses used. Notably, 2 animals died after treatment with the 2000 mg/kg dose, and one animal died in the 1000 mg/kg group. In the subacute toxicity study, neither male nor female rats inoculated with repeated doses of 8, 24, and 40mg/kg alpha compound showed significant changes in percent body weight (P = 0.5930). Among the females, significant differences were observed for the serum biochemical parameters glucose and cholesterol in the group inoculated with 8mg/kg compared to the control. The hematocrit values in the 8, 24 and 40mg/kg groups and the platelet count in the 24mg/kg group were significantly different (P = 0.0110) among the females compared to the control group. For the male rats, significant differences were found in erythrocyte count and mean corpuscular volume (MCV) in the groups treated with 8 and 40mg/kg. However, these values are normal according to the literature. The histopathological evaluations did not show apparent changes, so it is concluded that the alpha compound has a wide margin of safety after injection.
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