Characterization of commercial fibrolytic products for ruminant diets

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GERMÁN DAVID MENDOZA MARTÍNEZ
EMILIO ARANDA IBÁÑEZ
MARÍA MAGDALENA CROSBY GALVÁN
LUIS LANDOIS PALENCIA
LUIS ALBERTO MIRANDA ROMERO
LAURA RAMÍREZ CANCINO

Abstract

ENZYMATIC ACTIVITY (IU, INTERNATIONAL UNITS) WAS DETERMINED FOR THREE ENZYMATIC COMPLEXES (A, B, AND C), AT PH 5.5 AND 6.5, USING PH 102 CRYSTALLINE CELLULOSE, CARBOXYMETHYL CELLULOSE (CMC), AND OAT XYLAN AS SUBSTRATE. ACTIVITY WAS HIGHER (P < 0.05) AT PH 6.5. CELLULOLYTIC ACTIVITY WAS HIGHER (P < 0.05) WITH CRYSTALLINE CELLULOSE AND CMC WITH ENZYME A (53.68° AND 56.87°, IU). THE ACTIVITY WAS HIGHER (P < 0.05) WITH ENZYMES A AND B (222.16° Y 229.31°, UI) WITH XYLAN. THE DEGRADATION HALF TIME (H) WAS SIMILAR (P > 0.05) AMONG ENZYMES (A 4.02 H, B 4.18 HAND C 4.37 H). IN VITRO DIGESTION OF CRYSTALLINE CELLULOSE AND CMC WAS HIGHER (P < 0.05), WITH ENZYME C (75.69°%), AND FOR CMC WAS HIGHER WITH ENZYMES C AND B (99.9°% AND 87.04°B %) AND DIFFERENT BETWEEN ENZYMES C AND A (99.9°% AND 79.54B%). WHEN EXOGENOUS ENZYMES WERE INCUBATED WITH RUMINAL MICROBES, A HIGH ENZYMATIC ACTIVITY WITH PURIFIED SUBSTRATES WAS OBSERVED, WHICH DOES NOT NECESSARILY CORRESPONDS TO A HIGHEST DIGESTIBILITY, PRESUMABLY DUE TO THE DIFFERENCES ON THE DEGRADATION RATE AMONG THEM (A 17.22°%, B 16.57°B% AND C 15.85B%).
Keywords:
ENZYMES ACTIVITY CELLULOSE HEMICELLULOSE XYLAN

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