Assessment of the genetic variability in the captive population of Volcano Rabbit (Romerolagus diazi)
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Abstract
THE GENETIC VARIABILITY OF THE VOLCANO RABBIT (ROMEROLAGUS DIAZI) WAS ESTIMATED AND COMPARED AMONG INDIVIDUALS OF A WILD POPULATION AND A CAPTIVE ONE IN CHAPULTEPEC ZOO BY MEANS OF THE RAPD METHOD (RANDOM AMPLIFI ED POLYMORPHIC DNA). A TOTAL OF 31 DNA LOCI WERE AMPLIFIED, OBSERVING THAT BOTH POPULATIONS SHOWED GENETIC DIFFERENCES IN 8 LOCI (P < 0.05). THE WILD POPULATION PRESENTED A HIGHER AMOUNT OF GENETIC VARIABILITY WHEN COMPARED TO THE CAPTIVE POPULATION. OF THE 31 AMPLIFIED LOCI, 12 HAVE BECOME FI XED IN BOTH POPULATIONS, AND 10 ARE NOT STATISTICALLY SIGNIFI CANT BETWEEN POPULATIONS; IT WAS ALSO OBSERVED THAT IN THE CAPTIVE POPULATION SIX LOCI WERE LOST, TWO HAVE LOST 88% OF THEIR VARIABILITY AND ONE LOCUS PRESENTED A HIGHER AMOUNT OF VARIABILITY THAN THE WILD POPULATION.
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