Impact of bacillus and lactobacillus probiotics on growth performance, stress indices, and ileal histomorphology in broilers under high stocking density
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This study evaluated the impacts of bacillus and lactobacillus probiotics on broiler chicken growth performance, carcass traits, stress responses, and intestinal health at high stocking density. A total of 200 one-day-old broiler chicks (Cobb 500) were randomly assigned to five equal groups (4 replicates with 10 chicks per replicate) as follows: 1) LSD: low stocking density; 2) HSD: high stocking density; 3) HSDBacil: high stocking density with bacillus probiotic; 4) HSDLAB: high stocking density with lactobacillus probiotic; and 5) HSDMix: high stocking density with combined bacillus and lactobacillus probiotic. The LSD- and HSD-broilers were subjected to 12.5 and 16 birds/m2 stocking density, respectively. Growth performance, carcass traits, serum cortisol levels, oxidative stress markers, and ileal histomorphology were measured. The results indicated that body weight, body weight gain, and the weights of Fabricius bursa and breast meat were also adversely affected by high stocking density. Probiotic administration, particularly bacillus-based probiotic, increased body weight, body weight gain, and breast meat weight. Elevated serum cortisol and malondialdehyde levels, as well as decreased serum levels of total antioxidant capacity in the HSD group, confirmed the presence of heightened stress and oxidative damage, which were significantly reduced by probiotic administration. In addition, improvements in ileal histomorphology, including increased villus height and crypt depth, were observed in the probiotic-treated groups. Results indicated that the bacillus-based probiotic had a stronger impact on attenuating the undesirable effects of high stocking density in broilers and can be used as a beneficial nutritional additive against stressful conditions.
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Furnols MF. Meat consumption, sustainability, and alternatives: an overview of motives and barriers. Foods. 2023;12(11):2144. doi: 10.3390/foods12112144. DOI: https://doi.org/10.3390/foods12112144
Dal Bosco A, Mattioli S, Cartoni Mancinelli A, Cotozzolo E, Castellini C. Extensive rearing systems in poultry production: the right chicken for the right farming system. A review of twenty years of scientific research in Perugia University, Italy. Animals. 2021;11(5):1281. doi: 10.3390/ani11051281. DOI: https://doi.org/10.3390/ani11051281
Goo D, Kim JH, Park GH, Delos Reyes JB, Kil DY. Effect of heat stress and stocking density on growth performance, breast meat quality, and intestinal barrier function in broiler chickens. Animals. 2019;9(3):107. doi: 10.3390/ani9030107. DOI: https://doi.org/10.3390/ani9030107
Nasr MAF, Alkhedaide AQ, Ramadan AAI, Hafez ASE, Hussein MA. Potential impact of stocking density on growth, carcass traits, indicators of biochemical and oxidative stress, and meat quality of different broiler breeds. Poultry Science. 2021;100(5):101442. doi: 10.1016/j.psj.2021.101442. DOI: https://doi.org/10.1016/j.psj.2021.101442
Dai D, Qi G, Wang J, Zhang H, Qiu K, Han Y, et al. Dietary organic acids ameliorate high stocking density stress-induced intestinal inflammation through the restoration of intestinal microbiota in broilers. Journal of Animal Science and Biotechnology. 2022;13:124. doi: 10.1186/s40104-022-00776-2. DOI: https://doi.org/10.1186/s40104-022-00776-2
Royal Society for the Prevention of Cruelty to Animals. Welfare standards for meat chickens. West Sussex, UK (2017). https://business.rspcaassured.org.uk/media/qbuhlsvd/meat-chickens-standards-rspca.pdf
Zabir M, Miah MA, Alam M, Bhuiyan MEJ, Haque MI, Sujan KM, et al. Impacts of stocking density rates on welfare, growth, and hemato-biochemical profile in broiler chickens. Journal of Advanced Veterinary and Animal Research. 2021;8(4):642–649. doi: 10.5455/javar.2021.h556. DOI: https://doi.org/10.5455/javar.2021.h556
Kridtayopas C, Rakangtong C, Bunchasak C, Loongyai W. Effect of prebiotic and synbiotic supplementation in diet on growth performance, small intestinal morphology, stress, and bacterial population under high stocking density condition of broiler chickens. Poultry Science. 2019;98(10):4595–4605. doi: 10.3382/ps/pez152. DOI: https://doi.org/10.3382/ps/pez152
Ye Y, Li Z, Wang P, Zhu B, Zhao M, Huang D, et al. Effects of probiotic supplements on growth performance and intestinal microbiota of partridge shank broiler chicks. PeerJ. 2021;9:e12538. doi: 10.7717/peerj.12538. DOI: https://doi.org/10.7717/peerj.12538
Peng Q, Zeng XF, Zhu JL, Wang S, Liu XT, Hou CL, et al. Effects of dietary Lactobacillus plantarum B1 on growth performance, intestinal microbiota, and short-chain fatty acid profiles in broiler chickens. Poultry Science. 2016;95(4):893–900. doi: 10.3382/ps/pev435. DOI: https://doi.org/10.3382/ps/pev435
Jha R, Das R, Oak S, Mishra P. Probiotics (direct-fed microbials) in poultry nutrition and their effects on nutrient utilization, growth, and laying performance: a systematic review. Animals. 2020;10:1863. doi: 10.3390/ani10101863. DOI: https://doi.org/10.3390/ani10101863
Wu Y, Wang B, Zeng Z, Liu R, Tang L, Gong L, et al. Effects of probiotics Lactobacillus plantarum 16 and Paenibacillus polymyxa 10 on intestinal barrier function, antioxidative capacity, apoptosis, immune response, and biochemical parameters in broilers. Poultry Science. 2019;98:5028–5039. doi: 10.3382/ps/pez226 DOI: https://doi.org/10.3382/ps/pez226
Rasaei D, Hosseinian SA, Asasi K, Shekarforoush SS, Khodakaram-Tafti A. The beneficial effects of spraying of probiotic Bacillus and Lactobacillus bacteria on broiler chickens experimentally infected with avian influenza virus H9N2. Poultry Science. 2023;102(7):102669. doi: 10.1016/j.psj.2023.102669. DOI: https://doi.org/10.1016/j.psj.2023.102669
Khalid A, Khalid F, Mahreen N, Hussain SM, Shahzad MM, Khan S, Wang Z. Effect of spore forming probiotics on the poultry production: a review. Food Science of Animal Resources. 2022;42(6):968–980. doi: 10.5851/kosfa.2022.e41 DOI: https://doi.org/10.5851/kosfa.2022.e41
Cobb. Cobb 500. Broiler performance & nutrition supplement. 2022. https://www.cobb-vantress.com/assets/5a88f2e793/Broiler-Performance-Nutrition-Supplement.pdf
Ripplinger EN, Crespo R, Pullin AN, Carnaccini S, Nelson NC, Esteves PH, et al. Efficacy of a novel cervical dislocation tool for humane euthanasia of broilers and broiler breeders. Poultry Science. 2024;103(3):103449. doi: 10.1016/j.psj.2024.103449. DOI: https://doi.org/10.1016/j.psj.2024.103449
Bancroft JD, Layton C. The hematoxylins and eosin. In: Suvarna SK, Layton C, Bancroft JD, editors. Bancroft’s theory and practice of histological techniques. 7th edition. London:Churchill Livingstone, Elsevier; 2013. p. 173–187. DOI: https://doi.org/10.1016/B978-0-7020-4226-3.00010-X
Shynkaruk T, Long K, LeBlanc C, Schwean-Lardner K. Impact of stocking density on the welfare and productivity of broiler chickens reared to 34 d of age. Journal of Applied Poultry Research. 2023;32(2):100344. doi: 10.1016/j.japr.2023.100344. DOI: https://doi.org/10.1016/j.japr.2023.100344
Selvam R, Saravanakumar M, Suresh S, Sureshbabu G, Sasikumar M, Prashanth D. Effects of vitamin E supplementation and high stocking density on the performance and stress parameters of broilers. Brazilian Journal of Poultry Science. 2017;19(4):587–594. doi: 10.1590/1806-9061-2016-0417. DOI: https://doi.org/10.1590/1806-9061-2016-0417
Abudabos AM, Abdelrahman MM, Yehia HM, Al-Ghadi MQ, Alhidary IA. Effect of stocking density on intestinal histology and ileal bacterial count in broilers. Asian Journal of Poultry Science. 2013;8:740–746. doi: 10.3923/ajava.2013.740.746. DOI: https://doi.org/10.3923/ajava.2013.740.746
Siaga R, Baloyi JJ, Rambau MD, Benyi K. Effects of stocking density and genotype on the growth performance of male and female broiler chickens. Asian Journal of Poultry Science. 2017;11(2):96–104. doi: 10.3923/ajpsaj.2017.96.104. DOI: https://doi.org/10.3923/ajpsaj.2017.96.104
Lee KW, Lee SH, Lillehoj HS, Li GX, Jang SI, Babu US, et al. Effects of direct-fed microbials on growth performance, gut morphometry, and immune characteristics in broiler chickens. Poultry Science. 2010;89(2):203–216. doi: 10.3382/ps.2009-00418. DOI: https://doi.org/10.3382/ps.2009-00418
Wang Y, Gu Q. Effect of probiotic on growth performance and digestive enzyme activity of Arbor Acres broilers. Research in Veterinary Science. 2010;89(2):163–167. doi: 10.1016/j.rvsc.2010.03.009. DOI: https://doi.org/10.1016/j.rvsc.2010.03.009
Jayaraman S, Das PL, Saini PC, Roy B, Chatterjee PN. Use of Bacillus subtilis PB6 as a potential antibiotic growth promoter replacement in improving performance of broiler birds. Poultry Science. 2017;96(8):2614–2622. doi: 10.3382/ps/pex079. DOI: https://doi.org/10.3382/ps/pex079
Rivera-Pérez W, Barquero-Calvo E, Chaves AJ. Effect of the use of probiotic Bacillus subtilis (QST 713) as a growth promoter in broilers: an alternative to bacitracin methylene disalicylate. Poultry Science. 2021;100(9):101372. doi: 10.1016/j.psj.2021.101372. DOI: https://doi.org/10.1016/j.psj.2021.101372
Simitzis PE, Kalogeraki E, Goliomytis M, Charismiadou MA, Triantaphyllopoulos K, Ayoutanti A, et al. Impact of stocking density on broiler growth performance, meat characteristics, behavioural components, and indicators of physiological and oxidative stress. British Poultry Science. 2012;53(6):721–730. doi: 10.1080/00071668.2012.745930. DOI: https://doi.org/10.1080/00071668.2012.745930
Prokopidis K, Giannos P, Kirwan R, Ispoglou T, Galli F, Witard OC, et al. Impact of probiotics on muscle mass, muscle strength and lean mass: a systematic review and meta-analysis of randomized controlled trials. Journal of Cachexia, Sarcopenia and Muscle. 2023;4(1):30-44. doi: 10.1002/jcsm.13132. DOI: https://doi.org/10.1002/jcsm.13132
Krysiak K, Konkol D, Korczyński M. Overview of the use of probiotics in poultry production. Animals. 2021;11(6):1620. doi: 10.3390/ani11061620. DOI: https://doi.org/10.3390/ani11061620
Zhang ZF, Cho JH, Kim IH. Effects of Bacillus subtilis UBT-MO2 on growth performance, relative immune organ weight, gas concentration in excreta, and intestinal microbial shedding in broiler chickens. Livestock Science. 2013;155(2–3):343–347. doi: 10.1016/j.livsci.2013.05.021. DOI: https://doi.org/10.1016/j.livsci.2013.05.021
Mazziotta C, Tognon M, Martini F, Torreggiani E, Rotondo JC. Probiotics mechanism of action on immune cells and beneficial effects on human health. Cells. 2023;12(1):184. doi: 10.3390/cells12010184. DOI: https://doi.org/10.3390/cells12010184
Scanes CG. Biology of stress in poultry with emphasis on glucocorticoids and the heterophil to lymphocyte ratio. Poultry Science. 2016;95(9):2208–2215. doi: 10.3382/ps/pew137. DOI: https://doi.org/10.3382/ps/pew137
Mehaisen GMK, Eshak MG, Elkaiaty AM, Atta RMM, Mashaly MM, Abass AO. Comprehensive growth performance, immune function, plasma biochemistry, gene expressions and cell death morphology responses to a daily corticosterone injection course in broiler chickens. PLOS ONE. 2017;12:e0172684. doi: 10.1371/journal.pone.0172684. DOI: https://doi.org/10.1371/journal.pone.0172684
Hafez MH, El-Kazaz SE, Alharthi B, Ghamry HI, Alshehri MA, Sayed S, Shukry M, El-Sayed YS. The impact of curcumin on growth performance, growth-related gene expression, oxidative stress, and immunological biomarkers in broiler chickens at different stocking densities. Animals. 2022;12(8):958. doi: 10.3390/ani12080958. DOI: https://doi.org/10.3390/ani12080958
Oke OE, Akosile OA, Oni AI, Opowoye IO, Ishola CA, Adebiyi JO, et al. Oxidative stress in poultry production. Poultry Science. 2024;103(9):104003. doi: 10.1016/j.psj.2024.104003. DOI: https://doi.org/10.1016/j.psj.2024.104003
Chandrasekaran P, Weiskirchen S, Weiskirchen R. Effects of probiotics on gut microbiota: an overview. International Journal of Molecular Sciences. 2024;25(11):6022. doi: 10.3390/ijms25116022. DOI: https://doi.org/10.3390/ijms25116022
Surai PF. Antioxidant systems in poultry biology: Superoxide dismutase. Journal of Animal Research and Nutrition. 2016;1:8. doi: 10.21767/2572-5459.100008. DOI: https://doi.org/10.21767/2572-5459.100008
Li YQ, Zhang Y, Bai DY, Liu YH, He XL, Ito K, et al. Effects of dietary chlorogenic acid on ileal intestinal morphology, barrier function, immune factors and gut microbiota of broilers under high stocking density stress. Frontiers in Physiology. 2023;14:1169375. doi: 10.3389/fphys.2023.1169375. DOI: https://doi.org/10.3389/fphys.2023.1169375
Obianwuna UE, Agbai Kalu N, Wang J, Zhang H, Qi G, Qiu K, et al. Recent trends on mitigative effect of probiotics on oxidative-stress-induced gut dysfunction in broilers under necrotic enteritis challenge: a review. Antioxidants. 2023;12(4):911. doi: 10.3390/antiox12040911. DOI: https://doi.org/10.3390/antiox12040911
Shehata AA, Yalçın S, Latorre JD, Basiouni S, Attia YA, Abd El-Wahab A, et al. Probiotics, prebiotics, and phytogenic substances for optimizing gut health in poultry. Microorganisms. 2022;10(2):395. doi: 10.3390/microorganisms10020395. DOI: https://doi.org/10.3390/microorganisms10020395
Ducatelle R, Goossens E, Eeckhaut V, Van Immerseel F. Poultry health and beyond. Animal Nutrition. 2023;13:240–248. doi: 10.1016/j.aninu.2023.03.005. DOI: https://doi.org/10.1016/j.aninu.2023.03.005
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