Use of direct immunofluorescence and immunoperoxidase diagnostic kits for bovine parainfluenza 3 and bovine herpes virus 1 in Cuba

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MARITZA BARRERA VALLE
IRMA DELGADO DELGADO
SUSANA MENDOZA ELVIRA
BARRERO NORBIS RODRÍGUEZ
LILIAN SÁNCHEZ MIRANDA
ABEL CIPRIAN CARRASCO

Abstract

EARLY DIAGNOSIS IS ONE OF THE MAIN STRATEGIES IN PROGRAMS AGAINST DIFFERENT DISEASES THAT AFFECT HEALTH AND PRODUCTION OF LIVESTOCK. BOVINE HERPES VIRUS 1 (BHV-1) IS AN IMPORTANT CAUSE OF MORBIDITY AND MORTALITY IN CATTLE AND THE BOVINE PARAINFL UENZA 3 (PI-3) VIRUS CAUSES A FEBRILE, HIGHLY CONTAGIOUS PNEUMONIA OF ACUTE COURSE AFFECTING DIFFERENT TYPES OF CATTLE. DIRECT IMMUNOPEROXIDASE (DIP) AND DIRECT IMMUNOFL UORESCENCE (DIF) TESTS FOR THE DIAGNOSIS OF BHV-1 AND PI-3 IN NASAL SECRETIONS WERE STANDARDIZED AND ESTABLISHED AT CENSA. THE DIAGNOSTIC KITS WERE VALIDATED, INSPECTED AND REGISTERED. THE KITS WERE USEFUL TO DETECT VIRAL CONTAMINATION IN CELL CULTURES; AS WELL AS FOR THE IDENTIFICATION OF CALVES FREE OF PI-3 AND HPV-1, FOR DIAGNOSTIC SEVICES IN PREPUTIAL WASHES OF SEROREACTIVE OF CALVES BULLS DURING ARTIFICIAL INSEMINATION; AND FOR THE DIFFERENTIAL DIAGNOSIS OF VESICULAR DISEASES. SAMPLES WERE TAKEN FROM ANIMALS WIHT RESPIRATORY SIGNS TO DETERMINE THE ROLE OF THESE AGENTS IN THE ETIOLOGY OF PNEUMONIA CASES FROM HAVANA AND GRANMA PROVINCES. IT WAS FOUND THAT THE PI-3 VIRUS HAD GREATER INVOLVEMENT. THE KITS PROVED TO BE USEFUL FOR THEIR RAPIDITY, SENSITIVITY AND SPECIFICITY. THEY ARE LESS TIME CONSUMIG AND LESS EPNENSIVE THAN VIRAL INSOLATION. IT IS RECOMMENDED TO EXTEND THE USE OF THESE KITS IN ORDER TO DETERMINE THE ACTUAL ROLE OF BHV-I AND PI-3 VIRUSES IN DIFFERENT DISEASES FOUND IN THIS SPECIES, SINCE SCREENING FOR THESE VIRUSES WOULD BE DIFFICULT USIG VIRAL ISOLATION.
Keywords:
DIAGNOSTIC KITS IMMUNOPEROXIDASE IMMUNOFLUORESCENCE BHV-1 PI-3

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