Comparison between three diagnostic tests to detect abortion caused by infectious bovine rhinotracheitis in dairy herds

Abstract

THE PRESENT STUDY COMPARES THE SPECIFICITY (SP) AND SENSIBILITY (SE), AS WELL AS THE POSITIVE () AND NEGATIVE PREDICTIVE (""PV) VALUES OF IMMUNOPEROXIDASE (IP) AND IMMUNOFL UORESCENCE (IF) TESTS FOR THE DIAGNOSIS OF ABORTIONS CAUSED BY IBR, AS WELL AS THEIR CONCORDANCE WITH SERUM NEUTRALIZATION IN THE DAMS OF THE FETUSES, COMPARED AGAINST A STANDARD TEST OF VIRAL ISOLATION.(VI) FORTYNINE ABORTED FETUSES, OBTAINED OVER A ONE YEAR PERIOD, WERE EVALUATED. LIVER, SPLEEN, KIDNEY AND LUNG SAMPLES WERE COLLECTED FROM EACH FETUS. SERUM FROM THE DAMS WAS COLLECTED ON THREE OCCASIONS, ONE MONTH APART, STARTING IMMEDIATELY AFTER THE ABORTION. SAMPLES WERE TAKEN FROM ANIMALS IN ""AGRICULTURAL AND INDUSTRIAL COMPLEX OF TIZAYUCA (CAIT)"" TIZAYUCA, HIDALGO, AND THE DIAGNOSTIC TECHNIQUE WAS CARRIED OUT IN ""NATIONAL CENTER OF DIAGNOSTIC SERVICES IN ANIMAL HEALTH (CENASA)"" AS PRESCRIBED BY THE WORLD ORGANIZATION FOR ANIMAL HEALTH (OIE). A TOTAL OF 29 VIRAL ISOLATIONS OF IBR WERE OBTAINED. THE IMMUNOPEROXIDASE (IP) AND IMMUNOFL UORESCENCE (IF) TECHNIQUES HAD A SENSITIVITY OF 96.7% AND 24.1%, RESPECTIVELY, AND A SPECIFICITY OF 20% AND 70%, RESPECTIVELY. THE POSITIVE PREDICTIVE VALUES FOR THESE WERE 64.4% AND 53.8%, RESPECTIVELY, WHILE THE NEGATIVE PREDICTIVE VALUES WERE 80% AND 38.9%, RESPECTIVELY. THE CONCORDANCE FOR BOTH TESTS WAS BAD. OF THE ORGANS SAMPLED, THE LIVER AND KIDNEY WERE THE BEST FOR IF AND IP DIAGNOSIS OF IBR, RESPECTIVELY. CONCORDANCE FOR SERUM NEUTRALIZATION, IMMUNOFLUORESCENCE AND IMMUNOPEROXIDASE IN RELATION TO VIRAL ISOLATION WAS NOT SIGNIFICANT (P > 0.05).