Comparación de dos fuentes biológicas para reproducir anticuerpos destinados al desarrollo de métodos de radioinmunoanálisis para determinar los niveles plasmáticos de testosterona

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LUCIA ELIANA RANGEL PORTA
GERARDO PERERA MARÍN
ARANTZAZU IRUESTE LAS SALA
LUIS ZARCO

Abstract

THE PRESENT WORK COMPARED EGG YOLK AND RABBIT SERUM AS BIOLOGICAL SOURCES OF ANTIBODIES FOR THE DEVELOPMENT OF A RADIOIMMUNOASSAY (RIA) AGAINST TESTOSTERONE. IT WAS FOUND THAT AFTER TESTOSTERONE IMMUNIZATION, LAYING-HENS STOPPED PRODUCING EGGS APPARENTLY BECAUSE IMMUNIZATION AFFECTED EITHER THE FOLLICULAR DEVELOPMENT OR THE OVULATION PROCESS. IN CONTRAST, ANTIBODIES AGAINST TESTOSTERONE WERE OBTAINED IN THE RABBIT SERUM, AND A LIQUID PHASE RIA WAS DEVELOPED. RABBIT ANTIBODIES SHOWED HIGH SPECIFICITY, 5.84% CROSS-REACTING WITH DIHYDROTESTOSTERONE AND 0.72% WITH ANDROSTENEDIONE, ESTRADIOL AND 17-HYDROXIPROGESTERONE. SENSITIVITY AT 90% BINDING WAS 39.6 PG/ML. THE TOTAL ANTIBODY PRODUCTION WAS 126.083 MG, WHICH CAN BE USED TO PROCESS 1.6X105 SAMPLES. IN CONCLUSION, THE PRODUCTION OF ANTIBODIES AGAINST TESTOSTERONE IN EGG YOLK IS NOT RECOMMENDED AS AN ALTERNATIVE TO RABBIT SERUM, WHICH IS CONTRARY TO WHAT HAS BEEN FOUND FOR OTHER STEROID HORMONES.
Keywords:
ANTIBODIES TESTOSTERONE EGG YOLK RABBIT SERUM RADIOIMMUNOASSAY
DOI: https://doi.org/10.21753/vmoa.31.4.29

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