Kinetics of Mycoplasma hyopneumoniae experimental infection in pigs using immunofluorescence
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Abstract
BRONCHIAL EPITHELIUM FROM MYCOPLASMA HYOPNEUMONIAE EXPERIMENTALLY INFECTED PIGS WAS STUDIED BY USING IMMUNOFLUORESCENCE. COLONIZATION WAS ANALYZED DURING TWENTY DAYS POST-INFECTION. TWELVE ONE-MONTH-OLD PIGS WERE INTRATRACHEALLY INOCULATED WITH A MYCOPLASMA HYOPNEUMONIAE-INFECTED LUNG HOMOGENATE. PIGS WERE SACRIFICED ON DAYS 1, 4, 8, 12, 16 AND 20 POST-INFECTION, TWO EACH DAY; SIX CONTROL ANIMALS WERE INOCULATED WITH FRIIS MEDIA. PNEUMONIC LESIONS WERE EVALUATED AND ANALYZED BY M. HYOPNEUMONIAE ISOLATION AND HISTOLOGICAL STUDIES. DIRECT IMMUNOFLUORESCENCE WAS PERFORMED TO DETERMINED COLONIZATION. FLUORESCENCE WAS POSITIVE STARTING ON DAY FOUR; CILIUM CLUMPING WAS EVIDENT FROM DAY EIGHT. MYCOPLASMA INSTALLATION STARTED IN A SPOT OF THE BRONCHIA
Keywords:
KINETIC ENZOOTIC PNEUMONIA IMMUNOFLUORESCENCE INFECTION MYCOPLASMA HYOPNEUMONIAE PORCINE
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