Effect of two animal protein-free extenders on cryopreservation of Pelibuey and Blackbelly ram semen
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The study evaluated the effect of two animal protein-free extenders on ram semen cryopreservation of two tropical hair breeds. The ejaculates were collected from 8 rams (4 Blackbelly and 4 Pelibuey), which were mixed (pooled) by breed, diluted and cryopreserved in three different extenders: Animal protein [Tris egg yolk (Triladyl®)], and animal protein-free extenders AndroMed® (lecithin soy bean) and OPTIXcell® (liposome). Sperm analyses of total (TM) and progressive (PM) motility, viability, mitochondrial activity, acrosome integrity, and plasma membrane integrity (PMI) were carried out at 0 and 6 h after semen thawing. OPTIXcell® and Triladyl® extenders showed similar results between them and differed with AndroMed® in TM, PM, viability, and PMI (P<0.0017). In the Blackbelly breed, the TM was higher (P<0.0159) in OPTIXcell® than in AndroMed®. In the Pelibuey breed, the OPTIXcell® and Triladyl® showed similar results between them and differed with AndroMed® in TM, PM, viability, mitochondrial activity, and PMI (P<0.0140). However, Triladyl® showed a higher percentage of sperm with intact acrosome than AndroMed® (P<0.0392). In both breeds, spermatic parameters decreased progressively over the incubation time similarly in all three extenders. In conclusion, OPTIXcell® and Triladyl® proved to be the best extenders to cryopreserve Blackbelly and Pelibuey ram semen. However, OPTIXcell® is an animal protein-free extender that decreases the risk of bacterial contamination, whereas Triladyl® is composed of animal protein (egg yolk), which may impact the fertilizing capacity of sperm.
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Comizzoli P, Mermillod P, Mauget R. Reproductive biotechnologies for endangered mammalian species. Reproduction Nutrition Development. 2000;40(5):493–504. doi: 10.1051/rnd:2000113. DOI: https://doi.org/10.1051/rnd:2000113
Roca J, Rodríguez M, Gil M, Carvajal G, García E, Cuello C, et al. Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase. Journal of Andrology. 2005;26:15–24. PMID: 15611562. DOI: https://doi.org/10.1002/j.1939-4640.2005.tb02867.x
Viveiros A, Lock E, Woelders H, Komen J. Influence of cooling rates and plunging temperatures in an interrupted slow-freezing procedure for semen of the African catfish, Clarias gariepinus. Cryobiology. 2002;43(3):276–287. doi: 10.1006/cryo.2001.2362. DOI: https://doi.org/10.1006/cryo.2001.2362
Crespilho AM, Sá Filho MF, Dell’Aqua JA, Nichi M, Monteiro GA, Avanzi BR, et al. Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders. Livestock Science. 2012;149(1–2):1–6. doi: 10.1016/j.livsci.2012.05.011. DOI: https://doi.org/10.1016/j.livsci.2012.05.011
Huopalahti R, López-Fandiño R, Anton M, Schade R. Use of egg compounds for cryopreservation of spermatozoa. In: Bioactive Egg Compounds. Berlin, Heidelber: Springer-Verlag; 2007. 259–262 p. DOI: https://doi.org/10.1007/978-3-540-37885-3
Aires A, Hinsch K, Mueller F, Mueller-Schloesser F, Bogner K, Mueller-Schloesser S, et al. In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen. Theriogenology. 2003;60(2):269–279. doi: 10.1016/s0093-691x(02)01369-9. DOI: https://doi.org/10.1016/S0093-691X(02)01369-9
Amirat L, Anton M, Tainturier D, Chatagnon G, Battut I, Courtens JL. Modifications of bull spermatozoa induced by three extenders: Biociphos, low density lipoprotein and Triladyl, before, during and after freezing and thawing. Reproduction. 2005;129(4):535–543. doi: 10.1530/rep.1.00011. DOI: https://doi.org/10.1530/rep.1.00011
Yildiz C, Bozkurt Y, Yavas I. An evaluation of soybean lecithin as an alternative to avian egg yolk in the cryopreservation of fish sperm. Cryobiology. 2013;67(1):91–94. doi: 10.1016/j.cryobiol.2013.05.008. DOI: https://doi.org/10.1016/j.cryobiol.2013.05.008
Vidal AH, Batista AM, da Silva ECB, Gomes WA, Pelinca MA, Silva SV, et al. Soybean lecithin-based extender as an alternative for goat sperm cryopreservation. Small Ruminant Research. 2013;109(1):47–51. doi: 10.1016/j.smallrumres.2012.07.022. DOI: https://doi.org/10.1016/j.smallrumres.2012.07.022
El-Sisy GA, El-Nattat WS, El-Sheshtawy RI, El-Maaty AM. Substitution of egg yolk with different concentrations of soybean lecithin in tris-based extender during bulls semen preservability. Asian Pacific Journal Reproduction. 2016;5(6):514–518. doi: 10.1016/j.apjr.2016.10.011. DOI: https://doi.org/10.1016/j.apjr.2016.10.011
Marco-Jiménez F, Puchades S, Mocé E, Viudes-de-Cartro MP, Vicente JS, Rodriguez M. Use of powdered egg yolk vs fresh egg yolk for the cryopreservation of ovine semen. Reproduction in Domestic Animals. 2004;39(6):438–441. doi: 10.1111/j.1439-0531.2004.00537.x. DOI: https://doi.org/10.1111/j.1439-0531.2004.00537.x
Layek SS, Mohanty TK, Kumaresan A, Parks JE. Cryopreservation of bull semen: Evolution from egg yolk based to soybean based extenders. Animal Reproduction Science. 2016;172:1–9. doi: 10.1016/j.anireprosci.2016.04.013. DOI: https://doi.org/10.1016/j.anireprosci.2016.04.013
Ansari MS, Rakha BA, Andrabi SM, Akhter S. Usefulness of powdered and fresh egg yolk for cryopreservation of Zebu bull spermatozoa. Reproductive Biology. 2010;10(3):235–240. doi: 10.1016/s1642-431x(12)60043-6. DOI: https://doi.org/10.1016/S1642-431X(12)60043-6
Singh AK, Singh VK, Narwade BM, Mohanty TK, Atreja SK. Comparative quality assessment of buffalo (Bubalus bubalis) semen chilled (5 °C) in egg yolk and soya milk based extenders. Reprodution in Domestic Animals. 2012;47(4):596–600. doi: 10.1111/j.1439-0531.2011.01928.x. DOI: https://doi.org/10.1111/j.1439-0531.2011.01928.x
Pillet E, Duchamp G, Batellier F, Beaumal V, Anton M, Desherces S, et al. Egg yolk plasma can replace egg yolk in stallion freezing extenders. Theriogenology. 2011;75(1): 105–114. doi: 10.1016/j.theriogenology.2010.07.015. DOI: https://doi.org/10.1016/j.theriogenology.2010.07.015
Belala R, Delay J, Amirat L, Ropers MH, Guillou JL, Anton M, et al. The benefits of liposomes for chilling canine sperm for 4 days at 4 °C. Animal Reproduction Science. 2016;168:100–109. doi: 10.1016/j.anireprosci.2016.02.032. DOI: https://doi.org/10.1016/j.anireprosci.2016.02.032
Singh AK, Kumar A, Honparkhe M, Kaur S, Kaur H, Ghuman S, et al. Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk, soya bean lecithin and liposome based extenders. Reproduction in Domestic Animals. 2018;53(1):195–202. doi: 10.1111/rda.13092. DOI: https://doi.org/10.1111/rda.13092
Ansari MS, Rakha BA, Akhter S, Ashiq M. OPTIXcell improves the postthaw quality and fertility of buffalo bull sperm. Theriogenology. 2016;85(3):528–532. doi: 10.1016/j.theriogenology.2015.09.035. DOI: https://doi.org/10.1016/j.theriogenology.2015.09.035
INEGI. Anuario estadístico y geográfico de Yucatán; 2017.
Kvist U, Björndahl L. Sperm concentration. In: Manual on Basic Semen Analysis. Oxford: Oxford University Press; 2002. 7-9 p.
Nagy S, Jansen J, Topper EK, Gadella BM. A triple-stain flow cytometric method to assess plasma and acrosome membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biology Reproduction. 2003;68(5):1828–1835. doi: 10.1095/biolreprod.102.011445. DOI: https://doi.org/10.1095/biolreprod.102.011445
Mendoza C, Carreras A, Moos J, Tesarik J. Distinction between true acrosome reaction and degenerative acrosome loss by a one-step staining method using Pisum sativum agglutinin. Journal of Reproduction and Fertility. 1992;95(3):755–763. doi: 10.1530/jrf.0.0950755. DOI: https://doi.org/10.1530/jrf.0.0950755
Celeghini EC, De Arruda RP, De Andrade AF, Nascimento J, Raphael CF. Practical techniques for bovine sperm simultaneous fluorimetric assessment of plasma, acrosomal and mitochondrial membranes. Reproduction in Domestic Animals. 2007;42(5);479–488. doi: 10.1111/j.1439-0531.2006.00810.x. DOI: https://doi.org/10.1111/j.1439-0531.2006.00810.x
Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility. 1984;70(1):219–228. doi: 10.1530/jrf.0.0700219. DOI: https://doi.org/10.1530/jrf.0.0700219
Statistical Analysis System (SAS). Release 9.1 for windows. Cary, NC, USA: SAS Institute Inc; 2003.
Carrera-Chávez JM, Jiménez-Aguilar EE, Acosta-Pérez TP, Núñez-Gastélum JA, Quezada-Casasola A, Escárcega-Ávila AM, et al. Effect of Moringa oleifera seed extract on antioxidant activity and sperm characteristics in cryopreserved ram semen. Journal of Applied Animal Research. 2020;48(1):114–120. doi: 10.1080/09712119.2020.1741374. DOI: https://doi.org/10.1080/09712119.2020.1741374
Shehab-El-Deen M, Ali M, Al-Sharari M. Effects of extenders supplementation with gum arabic and antioxidants on ram spermatozoa quality after cryopreservation. Animals. 2023;13(1):111. doi: 10.3390/ani13010111. DOI: https://doi.org/10.3390/ani13010111
Moreira SSJ, Silva AM, Pereira AG, Santos RP, Dantas MRT, Souza-Júnior JBF, et al. Effect of detergents based on sodium dodecyl sulfate on functional metrics of frozen–thawed collared peccary (Pecari tajacu) semen. Animals. 2023;13(3):451. doi: 10.3390/ani13030451. DOI: https://doi.org/10.3390/ani13030451
Gutiérrez-Cepeda L, Crespo F, Blazquez JC, Serres C. Optimization of the equine-sperm freeze test in purebred Spanish horses by incorporating colloidal centrifugation. Animals. 2023;13(3):382. doi: 10.3390/ani13030382. DOI: https://doi.org/10.3390/ani13030382
Galián S, Peinado B, Almela L, Poto Á, Ruiz S. Post-thaw quality of spermatozoa frozen with three different extenders in the Murciano Granadina goat breed. Animals. 2023;13(2):309. doi: 10.3390/ani13020309. DOI: https://doi.org/10.3390/ani13020309
Ectors FJ, Vanderzwalmen P, Van Hoeck J, Nijs M, Verhaegen G, Delvigne A, et al. Relationship of human follicular diameter with oocyte fertilization and development after in-vitro fertilization or intracytoplasmic sperm injection. Human Reproduction. 1997;12(9):2002–2005. doi: 10.1093/humrep/12.9.2002. DOI: https://doi.org/10.1093/humrep/12.9.2002
Swain JE, Pool TB. ART failure: oocyte contributions to unsuccessful fertilization, Human Reproduction Update. 2008;14(5):431–446. doi: 10.1093/humupd/dmn025. DOI: https://doi.org/10.1093/humupd/dmn025
Anel L, Alvarez M, Martinez-Pastor F, Garcia-Macias V, Anel E, De Paz P. Improvement strategies in ovine artificial insemination. Reproduction in Domestic Animals. 2006;41(s2):30–42. doi: 10.1111/j.1439-0531.2006.00767.x. DOI: https://doi.org/10.1111/j.1439-0531.2006.00767.x
Dixon AB, Knights M, Winkler JL, Marsh DJ, Pate JL, Wilson ME, et al. Patterns of late embryonic and fetal mortality and association with several factors in sheep. Journal of Animal Science. 2007;85(5):1274–1284. doi: 10.2527/jas.2006-129. DOI: https://doi.org/10.2527/jas.2006-129
Gharibzadeh Z, Riasi A, Ostadhosseini S, Hosseini SM, Hajian M, Nasr-Esfahani MH. Effects of heat shock during the early stage of oocyte maturation on the meiotic progression, subsequent embryonic development and gene expression in ovine. Zygote. 2015;23(4):573–582. doi: 10.1017/S0967199414000203. DOI: https://doi.org/10.1017/S0967199414000203
Abecia JA, Arrébola F, Macías A, Laviña A, González-Casquet O, Benítez F, Palacios C. Temperature and rainfall are related to fertility rate after spring artificial insemination in small ruminants. International Journal of Biometeorology. 2016;60:1603–1609. doi: 10.1007/s00484-016-1150-y. DOI: https://doi.org/10.1007/s00484-016-1150-y
Errandonea N, Fierro S, Viñoles C, Gil J, Banchero G, Olivera-Muzante J. Short term protein supplementation during a long interval prostaglandin-based protocol for timed AI in sheep. Theriogenology. 2018;114(1):34–39. doi: 10.1016/j.theriogenology.2018.03.021. DOI: https://doi.org/10.1016/j.theriogenology.2018.03.021
Gibbons AE, Fernandez J, Bruno-Galarraga MM, Spinelli MV, Cueto MI. Technical recommendations for artificial insemination in sheep. Animal Reproduction. 2019;16(4):803–809. doi: 10.21451/1984-3143-AR2018-0129. DOI: https://doi.org/10.21451/1984-3143-AR2018-0129
Priskas S, Termatzidou SA, Gargani S, Arsenos G. Evaluation of factors affecting pregnancy rate after cervical insemination of dairy ewes in Greece. Journal of Veterinary Science and Medicine. 2019;7(2):1–7. DOI: https://doi.org/10.13188/2325-4645.1000047
Santolaria P, Vicente-Fiel S, Palacín I, Fantova E, Blasco ME, Silvestre MA, et al. Predictive capacity of sperm quality parameters and sperm subpopulations on field fertility after artificial insemination in sheep. Animal Reproduction Science. 2015;163:82–88. doi: 10.1016/j.anireprosci.2015.10.001. DOI: https://doi.org/10.1016/j.anireprosci.2015.10.001
Christensen P, Boelling D, Pedersen KM. Korsgaard IR, Jensen J. Relationship between sperm viability as determined by flow cytometry and nonreturn rate of dairy bulls. Journal of Andrology. 2005;26(1):98–106. doi: 10.1002/j.1939-4640.2005.tb02878.x. DOI: https://doi.org/10.1002/j.1939-4640.2005.tb02878.x
Januskauskas A, Johannisson A, Rodriguez-Martinez H. Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure and field fertility. Theriogenology 2003;60(4):743–758. doi: 10.1016/s0093-691x(03)00050-5. DOI: https://doi.org/10.1016/S0093-691X(03)00050-5
Tartaglione CM, Ritta MN. Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen–thawed bull semen. Theriogenology 2004;62(7):1245–1252. doi: 10.1016/j.theriogenology.2004.01.012. DOI: https://doi.org/10.1016/j.theriogenology.2004.01.012
Rodríguez-Martinez H. State of art in farm animal sperm evaluation. Reproduction, Fertility and Development. 2007;19(1):91–101. doi: 10.1071/rd06104. DOI: https://doi.org/10.1071/RD06104
Christensen P, Labouriau R, Birck A, Boe-Hansen GB, Pedersen J, Borchersen S. Relationship among seminal quality measures and field fertility of young dairy bulls using low-dose inseminations. Journal of Dairy Science. 2011;9484):1744–1754. doi: 10.3168/jds.2010-3087. DOI: https://doi.org/10.3168/jds.2010-3087
Kumaresan A, Johannisson A, Al-Essawe EM, Morrell JM. Sperm viability, reactive oxygen species, and DNA fragmentation index combined can discriminate between above- and below-average fertility bulls. Journal of Dairy Science. 2017;100(7):5824–5836. doi: 10.3168/jds.2016-12484. DOI: https://doi.org/10.3168/jds.2016-12484
Samplaski MK, Dimitromanolakis A, Lo KC, Grober ED, Mullen B, Garbens A, Jarvi KA. The relationship between sperm viability and DNA fragmentation rates. Reproductive Biology and Endocrinology. 2015;14:13-42. doi: 10.1186/s12958-015-0035-y. DOI: https://doi.org/10.1186/s12958-015-0035-y
Van de Hoek M, Rickard JP, de Graaf SP. Motility assessment of ram spermatozoa. Biology. 2022;11(12):1715. doi: 10.3390/biology11121715. DOI: https://doi.org/10.3390/biology11121715
Kjaestad H, Ropstad E, Berg KA. Evaluation of spermatological parameters used to predict the fertility of frozen bull semen. Acta Veterinaria Scandinavica. 1993;34(3):299–303. doi: 10.1186/BF03548194. DOI: https://doi.org/10.1186/BF03548194
Wierzbowski S, Kareta W. An assessment of sperm motility estimation for evaluation in rams. Theriogenology. 1993;40(1):205–209. doi: 10.1016/0093-691X(93)90354-8. DOI: https://doi.org/10.1016/0093-691X(93)90354-8
Love CC. Relationship between sperm motility, morphology and the fertility of stallions. Theriogenology 2011;76(3):547–557. doi: 10.1016/j.theriogenology.2011.03.007. DOI: https://doi.org/10.1016/j.theriogenology.2011.03.007
Puglisi R, Pozzi A, Foglio L, Spanò M, Eleuteri P, Grollino MG, et al. The usefulness of combining traditional sperm assessments with in vitro heterospermic insemination to identify bulls of low fertility as estimated in vivo. Animal Reproduction Science. 2012;132(1-2):17–28. doi: 10.1016/j.anireprosci.2012.04.006. DOI: https://doi.org/10.1016/j.anireprosci.2012.04.006
David I, Kohnke P, Lagriffoul G, Praud O, Plouarboué F, Degond P, et al. Mass sperm motility is associated with fertility in sheep. Animal Reproduction Science. 2015;161:75-81. doi: 10.1016/j.anireprosci.2015.08.006. DOI: https://doi.org/10.1016/j.anireprosci.2015.08.006
Fernández-López P, Garriga J, Casas I, Yeste M, Bartumeus F. Predicting fertility from sperm motility landscapes. Communications Biology. 2022;5:1027. doi: 10.1038/s42003-022-03954-0. DOI: https://doi.org/10.1038/s42003-022-03954-0
Villani MT, Morini D, Spaggiari G, Falbo AI, Melli B, La Sala GB, et al. Are sperm parameters able to predict the success of assisted reproductive technology? A retrospective analysis of over 22,000 assisted reproductive technology cycles. Andrology. 2022;10(2):310–321. doi: 10.1111/andr.13123. DOI: https://doi.org/10.1111/andr.13123
Malić Vončina S, Golob B, Ihan A, Kopitar AN, Kolbezen M, Zorn B, et al. Sperm DNA fragmentation and mitochondrial membrane potential combined are better for predicting natural conception than standard sperm parameters. Fertility and Sterility. 2016;105(3):637-644. doi: 10.1016/j.fertnstert.2015.11.037. DOI: https://doi.org/10.1016/j.fertnstert.2015.11.037
Ferreira JJ, Cassina A, Irigoyen P, Ford M, Pietroroia S, Peramsetty N, et al. Increased mitochondrial activity upon CatSper channel activation is required for mouse sperm capacitation. Redox Biology. 2021;48. No. 102176. doi: 10.1016/j.redox.2021.102176. DOI: https://doi.org/10.1016/j.redox.2021.102176
Giaccagli MM, Gómez-Elías MD, Herzfeld JD, Marín-Briggiler CI, Cuasnicú PS, Cohen DJ, et al. Capacitation-induced mitochondrial activity is required for sperm fertilizing ability in mice by modulating hyperactivation. Frontiers in Cell and Developmental Biology. 2021;9. No. 767161. doi: 10.3389/fcell.2021.767161. DOI: https://doi.org/10.3389/fcell.2021.767161
Brito LFC, Barth AD, Bilodeau-Goeseels S, Panich PL,Kastelic JP. Comparison of methods to evaluate the plasmalemma of bovine sperm and their relationship with in vitro fertilization rate. Theriogenology. 2003;60(8):1539–1551. doi: 10.1016/s0093-691x(03)00174-2. DOI: https://doi.org/10.1016/S0093-691X(03)00174-2
Oliveira LZ, de Arruda RP, de Andrade AFC, Celeghini ECC, dos Santos RM, Beletti ME, et al. Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cows. Livestock Science. 2012;146(1):38–46. doi: 10.1016/j.livsci.2012.02.018. DOI: https://doi.org/10.1016/j.livsci.2012.02.018
Liu DY, Baker HW. Calcium ionophore-induced acrosome reaction correlates with fertilization rates in vitro in patients with teratozoospermic semen. Human Reproduction. 1998;13(4):905–910. doi: 10.1093/humrep/13.4.905. DOI: https://doi.org/10.1093/humrep/13.4.905
Xu F, Zhu H, Zhu W, Fan L. Human sperm acrosomal status, acrosomal responsiveness, and acrosin are predictive of the outcomes of in vitro fertilization: A prospective cohort study. Reproductive Biology. 2018;18(4):344–354. doi: 10.1016/j.repbio.2018.10.007. DOI: https://doi.org/10.1016/j.repbio.2018.10.007
Stewart JL, Shipley CF, Katich AS, Po E, Ellerbrock RE, Lima FS, et al. Cryopreservation of white-tailed deer (Odocoileus virginianus) semen using soybean, liposome and egg yolk-based extenders. Animal Reproduction Science. 2016;171:7–16. doi: 10.1016/j.anireprosci.2016.05.006. DOI: https://doi.org/10.1016/j.anireprosci.2016.05.006
Murphy EM, O’Meara C, Eivers B, Lonergan P, Fair S. Comparison of plant and egg yolk-based semen diluents on in vitro sperm kinematics and in vivo fertility of frozen-thawed bull semen. Animal Reproduction Science. 2018;191:70–75. doi: 10.1016/j.anireprosci.2018.02.010. DOI: https://doi.org/10.1016/j.anireprosci.2018.02.010
Lima-Verde IB, Johannisson A, Ntallaris T, Al-Essawe E, Al-Kass Z, Nongbua T, et al. Effect of freezing bull semen in two non-egg yolk extenders on post-thaw sperm quality. Reproduction in Domestic Animals. 2018;53(1):127–136. doi: 10.1111/rda.13080. DOI: https://doi.org/10.1111/rda.13080
Kang SS, Lee MS, Kim UH, Lee SD, Yang BC, Yang BS, et al. Effect of Optixcell and Triladyl extenders on frozen-thawed sperm motilities and calving rates following artificial insemination in Hanwoo. Korean Journal Agricultural Science. 2019;46(1);195–204. doi: 10.7744/kjoas.20190009. DOI: https://doi.org/10.7744/kjoas.20190009
Abdel-Aziz SA, Saadeldin IM, Ba-Awadh H, Al-Mutary MG, Moumen AF, Alowaimer AN, et al. Efficiency of commercial egg-yolk free and egg-yolk supplemented tris-based extenders for dromedary camel semen cryopreservation. Animals. 2019;9(11):999. doi: 10.3390/ani9110999. DOI: https://doi.org/10.3390/ani9110999
Ondřej Š, Jiří Š, Jan B, Pavla MP, Lucie T, Doležalová M, et al. Low density lipoprotein important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders. Animal Reproduction. 2019;16(2):267–276. doi: 10.21451/1984-3143-AR2018-0107. DOI: https://doi.org/10.21451/1984-3143-AR2018-0107
Mehdipour M, Daghigh Kia H, Nazari M, Najafi A. Effect of lecithin nanoliposome or soybean lecithin supplemented by pomegranate extract on post-thaw flow cytometric, microscopic and oxidative parameters in ram semen. Cryobiology. 2017;78:34–40. doi: 10.1016/j.cryobiol.2017.07.005. DOI: https://doi.org/10.1016/j.cryobiol.2017.07.005
Luna-Orozco JR, González-Ramos MA, Calderón-Leyva G, Gaytán-Alemán LR, Arellano-Rodríguez F, Ángel-García O, et al. Comparison of different diluents based on liposomes and egg yolk for ram semen cooling and cryopreservation. Iranian Journal of Veterinary Research. 2019;20(2):126–130. PMCID: PMC6716279.
Wojtusik J, Stoops MA, Roth TL. Animal protein-free OptiXcell and shortened equilibration periods can replace egg yolk-based extender and slow cooling for rhinoceros semen cryopreservation. Cryobiology. 2019;89:21–25. doi: 10.1016/j.cryobiol.2019.06.003. DOI: https://doi.org/10.1016/j.cryobiol.2019.06.003
Amal AS, Arifiantini RI, Setiadi MA, Said S. Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times. Journal of the Indonesian Tropical Animal Agriculture. 2019:44(2);135-145. doi: 10.14710/jitaa.44.2.135-145. DOI: https://doi.org/10.14710/jitaa.44.2.135-145
Singh A, Bhakat M, Mohanty TK, Mondal S, Yadav SK, Kumar P, et al. Effect of tris-egg yolk, soya milk and liposome based extenders on sahiwal (Bos indicus) sperm quality during pre and post cryopreservation stages. CryoLetters. 2019;40(2):94–102. PMID: 31017609.
Gomes-Alves S, Alvarez M, Nicolas M, Lopez-Urueña E, Martínez-Rodríguez C, Borragan S, et al. Use of commercial extenders and alternatives to prevent sperm agglutination for cryopreservation of brown bear semen. Theriogenology. 2014;82(3):469–474. doi: 10.1016/j.theriogenology.2014.05.015. DOI: https://doi.org/10.1016/j.theriogenology.2014.05.015
Fleisch A, Malama E, Witschi U, Leiding C, Siuda M, Janett F, et al. Effects of an extension of the equilibration period up to 96 hours on the characteristics of cryopreserved bull semen. Theriogenology. 2017:89;255–262. doi: 10.1016/j.theriogenology.2016.10.018. DOI: https://doi.org/10.1016/j.theriogenology.2016.10.018
Souza C, Brandao F, Santos J, Alfradique V, Brair V, Prellwitz L, et al. 38 Ram sperm longevity after cryopreservation in extender containing L-carnitine. Reproduction, Fertility and Development. 2019;32(2):145. doi: 10.1071/RDv32n2Ab38. DOI: https://doi.org/10.1071/RDv32n2Ab38
Loomis PR, Graham JK. Commercial semen freezing: individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Animal Reproduction Science. 2008;105(1–2):119–128. doi: 10.1016/j.anireprosci.2007.11.010. DOI: https://doi.org/10.1016/j.anireprosci.2007.11.010
Arav A, Pearl M, Zeron Y. Does membrane lipid profile explain chilling sensitivity and membrane lipid phase transition of spermatozoa and oocytes? CryoLetters. 2000;21(3):179–186. PMID: 12148049.
Holt WV. Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology. 2000;53(1):47–58. doi: 10.1016/S0093-691X(99)00239-3. DOI: https://doi.org/10.1016/S0093-691X(99)00239-3
Waterhouse KE, Hofmo PO, Tverdal A, Miller RR. Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm. Reproduction. 2006;131(5):887–894. doi: 10.1530/rep.1.01049 DOI: https://doi.org/10.1530/rep.1.01049
Kumar P, Saini M, Kumar D, Balhara AK, Yadav SP, Singh P, et al. Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Animal Reproduction Science. 2015;159:38–45. doi: 10.1016/j.anireprosci.2015.05.010. DOI: https://doi.org/10.1016/j.anireprosci.2015.05.010
Talini R, Kozicki LE, Gaievski FR, Polo G, Lima LG, Santiago J, et al. Bovine semen thermoresistance tests and their correlation with pregnancy rates after fixed-time artificial insemination. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2019;71(06):1917–1925. doi: 10.1590/1678-4162-10994. DOI: https://doi.org/10.1590/1678-4162-10994
Naijian HR, Kohram H, Shahneh AZ, Sharafi M. Effects of various concentrations of BSA on microscopic and oxidative parameters of Mahabadi goat semen following the freeze–thaw process. Small Ruminant Research. 2013:113(2);371–375. doi: 10.1016/j.smallrumres.2013.03.015. DOI: https://doi.org/10.1016/j.smallrumres.2013.03.015
Salmin S, Ismaya I, Kustopo K, Beliarti E. The effect of semen washing and soybean lecithin level on motility and viability of ram spermatozoa stored at 5 °C. Journal of the Indonesian Tropical Animal Agriculture. 2012;37(4):244–249. doi: 10.14710/jitaa.37.4.244-249. DOI: https://doi.org/10.14710/jitaa.37.4.244-249
Asadpour R, Jafari R, Tayefi-Nasrabadi H. The effect of antioxidant supplementation in semen extenders on semen quality and lipid peroxidation of chilled bull spermatozoa. Iranian Journal of Veterinary Research. 2012;13(3):246–249. doi: 10.22099/IJVR.2012.365.
Pillet E, Labbe C, Batellier F, Duchamp G, Beaumal V, Anton M, et al. Liposomes as an alternative to egg yolk in stallion freezing extender. Theriogenology. 2012;77(2): 268–279. doi: 10.1016/j.theriogenology.2011.08.001. DOI: https://doi.org/10.1016/j.theriogenology.2011.08.001
Belala R, Briand-Amirat L, Vinciguerra L, Tainturier D, Kaidi R, Thorin C, et al. Effect of equilibration time on the motility and functional integrity of canine spermatozoa frozen in three different extenders. Research in Veterinary Science. 2016;106:66–73. doi: 10.1016/j.rvsc.2016.03.010. DOI: https://doi.org/10.1016/j.rvsc.2016.03.010
Sieme H, Oldenhof H, Wolkers WF. Sperm membrane behaviour during cooling and cryopreservation. Reproduction in Domestic Animals. 2015;50(3):20–26. doi: 10.1111/rda.12594. DOI: https://doi.org/10.1111/rda.12594
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